Bone Abstracts

Osteoclasts are multinucleated bone-resorbing cells and can be cultured from different monocytic precursors. It is unknown whether osteoclasts derived from different precursors are phenotypically different. One of the aspects not known is the life span of the different osteoclasts and the effect of IL-1β hereupon. Here, we studied this using time-lapse microscopy. Bone marrow cells were isolated from 6-week-old male mice. Early blasts (CD31hiLy-6C-),...

Calcium supplements are associated with increased cardiovascular risk, but the mechanism by which this occurs is presently uncertain. In a secondary analysis of a trial examining the acute effects of calcium supplements, we found that blood pressure declined over 8 h in the control group, consistent with its diurnal rhythm, and that this decline was smaller in the calcium group [1]. To investigate these effects further, we carried out a randomised controlled cross-over trial o...

Vertebral fractures (VF) defined by morphometric analysis of spine radiographs are the most common complication of osteoporosis. Those that come to medical attention, with symptoms such as back pain and kyphosis are termed clinical vertebral fractures (CVF) and account for significant morbidity and mortality. Although much progress was made in identifying loci for bone mineral density, the genetic determinants of CVF remain unclear. Here we present the initial results from a g...

Aim: To determine the safety, tolerability, pharmacokinetics and pharmacodynamics of the cathepsin K inhibitor MIV-711.Methods: A double-blind, placebo-controlled, randomized study in 27 healthy subjects of both genders. Single ascending doses 20–600 mg were investigated for adverse events, clinical chemistry, vital signs, ECG parameters, pharmacokinetics, and serum levels of CTX-I.Results: MIV-711 was well tolerated with no a...

Recombinant human parathyroid hormone (rhPTH) (1–84) is currently being developed as PTH replacement therapy for patients with hypoparathyroidism. Because rhPTH(1–84) is administered subcutaneously, we compared the pharmacokinetic profile of PTH(1–84) and the calcemic response following S.C. rhPTH(1–84) injection in thigh and abdomen.In this phase 1, open-label, three-way crossover study, healthy postmenopausal women received three ra...

Purpose: To investigate the effect of enamel matrix derivative (Emdogain) on the attachment, growth behavior and the genetic effect of human gingival fibroblasts (HGF) cultured on zirconium disc surfaces.Materials and methods: HGF cells were cultured on i) zirconium discs without enamel matrix derivative (EMD), ii) zirconium discs with EMD 25 μg/ml, and iii) zirconium discs with EMD 100 μg/ml. The cell proliferation activity was evaluated throu...

Bone is a complex organ constituted of a mineralized matrix generated by osteoblasts (OB). Bone matrix is a major storage site for minerals but also for toxicants from the environment. Among them, uranium, a natural element of the earth crust, has a dual toxicity due to its radiological effects as an alpha emitter and its chemical effects due to its metal properties. In the case of natural uranium, the chemical toxicity is predominant. Uranium level in drinking water is usuall...

Objectives: Children with Duchenne muscular dystrophy (DMD) receiving long-term glucocorticoid (GC) therapy are at risk for osteoporosis and fragility fractures. Recent studies showed that cortical thickness and areas were associated with increased fracture risk. Digital X-ray measurement of the cortical thickness of the metacarpal bones has a potential role as a marker for bone health in children, but has not been evaluated in DMD. The aim of this study was to compare bone ag...

Objective: We investigated the effect of arginine administered for 10 weeks in diet on bone metabolism in male Wistar rats.Methods: Rats were divided (after approval of Ethical Committee 9814/2008-30 and 30793/2010-30) into four groups, ten animals in each group: G1 SLD-S group were fed a standard laboratory diet (SLD). G2 SLD-H group were fed SLD and 12 h before rats were sacrificed diet was removed. G3 ARG-S group was fed wit...

IGF1 is an osteo-anabolic factor that stimulates osteogenic precursor cell differentiation. IGF1 is produced in bone in response to mechanical stimulation, but also in mechanically-stimulated muscle cells. IGF1 enhances the rate of mRNA translation in muscle cells via activation of the PI3K/AKT/mTOR pathway, thereby increasing in muscle mass. Therefore we hypothesized that IGF1 not just enhances osteogenic differentiation of precursors, but also stimulates protein syn...