Bone Abstracts

Bone sialoprotein regulates osteoblast activity and bone formation. In knockout (BSP−/−) mouse bone marrow (BM) stromal cell cultures, the pool of osteoprogenitor (OP) cells (CFU-F number) is not different from wild-type (+/+), nor is their early differentiation (same numbers of alkaline phosphatase positive colonies=CFU-ALP, although these are smaller), while the number of osteoblast, mineralized colonies (CFU-OB) is dramatically reduced. Because ossifi...

Despite its importance, the resorptive surface of osteoclasts has not been directly visualised. We exploited a novel approach that enables us to inspect the substrate-apposed surface of cells. To achieve this, we incubated osteoclasts on vitronectin-coated nail varnish and, afterwards, we dissolved the substrate and visualised the cells resorbing-side up in the scanning electron microscope (SEM). We then employed confocal microscopy to correlate the SEM appearances with the di...

Background: Vibration analysis is a well-established technique in industry to analyse materials physical properties. The application to bone’s physical properties is unclear. This study investigated the relationship between bone vibration frequency and mass:volume ratio (ρ).Methods: We used eight turkey bones (tibio tursus). Following soft tissue removal, a 12 cm diaphyseal section was isolated, marrow removed using a water jet and the...

Objective: The enzyme methylenetetrahydrofolate reductase (MTHFR) is known to play an important role in the removal of circulating homocysteine via the methionine cycle. C677T polymorphism is associated with higher plasma homocysteine levels, which could affect collagen maturation. The aim of the present study was to examine possible associations of C677T polymorphism in the MTHFR gene with a variability of femoral (F-BMD), spinal BMD (S-BMD) together with circulating...

Objective: Currently, majority of the osteoporotic patients are not diagnosed i) a new ultrasound based device (Bindex) has been recently introduced for osteoporosis (OP) screening and diagnostics at primary healthcare ii) Bindex measures cortical thickness and determines parameter called density index (DI). Thresholds for DI in OP assessment have been determined in Finnish-Caucasian (F-C) population (n=448) along the International Society of Clinical Densitometry (IS...

Background: There is significant inter and intraobserver variability in diagnosing vertebral fractures in children. We aimed to evaluate the diagnostic accuracy of morphometric vertebral fracture analysis (MXA) using a 33-point software programme designed for adults, on dual-energy x-ray absorptiometry (DXA) images of children.Methods: Lateral spine DXA images of 420 children aged between 5 and 18 years were retrospectively reviewed. Vertebral fracture a...

The aim of this study was to evaluate the trabecular bone score (TBS), bone mineral density (BMD) and body composition in men of various ages.Materials and methods: 300 men aged 40–87 years (mean age – 60.5±0.6 years; mean height – 1.61±0.003 m; mean weight – 84.1±0.9 kg) were examined. The patients were divided into the following age-dependent groups: 40–49 years (n=52), 50–59 years (n=90), ...

The aim of this study was to evaluate the trabecular bone score (TBS), bone mineral density (BMD) and body composition in women of various ages.Materials and methods: 494 women aged 41–89 years (mean age, 63.6±0.4 years; mean height, 1.61±0.003 m; and mean weight, 74.0±0.6 kg) were examined. The patients were divided into the following age-dependent groups: 40–49 years (n=35), 50–59 years (n=130), 60–6...

The aim of the study was to evaluate the role of vitamin D and exercises in correction of age-related skeletal muscle changes in postmenopausal women.Materials and methods: 38 postmenopausal women aged 53–82 years (mean age – 67.00±7.08 yrs) were examined. The women were divided into the following groups: A – control group (n=10), B – women who took an individually-targeted vitamin D therapy (n=11), C – wom...

Arterial calcification is now considered as an actively regulated process resembling osteogenesis orchestrated by a number of systemic or constitutively expressed mediators. Genetic studies of rare inherited syndromes have identified key regulators of arterial calcification. Based on the pathogenic principles causing the diseases these can be classified into three groups:i) Disorders of an increased extracellular inorganic phosphate/inorganic pyrophospha...