Bone Abstracts

Background: Zoledronic acid (ZA) is associated with a transient post-infusional acute phase reaction (APR) due to the activation of γδTCR lymphocytes (γδTcells).Aims: To investigate if APR correlates with γδTcell percentage or cytokine polarization, or with 25-OH vitamin D levels; to identify a 25-OH vitamin D level associated with a lower risk of APR.Methods: Sera for ELISA test (IFNγ, IL17, and ...

Long-term use of pharmacological treatments of osteoporosis is currently a controversial subject within the scientific community.The aim of this work was to study the effects produced by a preventive long duration treatment of strontium ranelate and zoledronic acid on bone mass and remodelling when they are administered to ovariectomized rats.Sixty female albino Wistar rats were divided into four groups (n=15): SHAM group ...

Prostate cancer (PCa) is the most common cancer in males. When patients develop metastasis, no curative therapy is available. To find new therapeutic options, it is crucial to understand how PCa cells induce metastasis. Recently, it was shown that PCa cells secrete small extracellular vesicles (EVs) that can be found in the circulation and in bones. Uptake of EVs by other cells may change their behaviour. We previously identified three miRNAs that were uniquely upregulated in ...

Calcium supplements are associated with increased cardiovascular risk, but the mechanism by which this occurs is presently uncertain. In a secondary analysis of a trial examining the acute effects of calcium supplements, we found that blood pressure declined over 8 h in the control group, consistent with its diurnal rhythm, and that this decline was smaller in the calcium group [1]. To investigate these effects further, we carried out a randomised controlled cross-over trial o...

see P260....

Background and Objectives: Rheumatoid arthritis (RA) is a chronic disease characterized by severe bone destruction which has been associated with altered lipid metabolism. Apolipoprotein E (Apo E) is a lipoprotein mainly produced by macrophages. ApoE has been described as crucial in lipid metabolism but also as an important anti-inflammatory mediator regulating innate immunity and bone turnover. In the present study we investigated the role of Apo E in bone destruction during ...

Dietary Ca insufficiency is a common finding, independently of socio-economic status. A low Ca intake (CaI) increases bone turnover leading to bone loss. This effect becomes more important during estrogen withdrawing. We previously found that a mixture of galactooligosaccharides (GOS) and fructooligosaccharides (FOS) enhances Ca and P absorption (Abs), being a suitable tool to optimize its bioavailability and consequently bone health. The present study evaluated the effect of ...

Titanium (Ti) surface with nanotopography induces osteoblast differentiation, at least in part, by upregulating the expression of α1/β1 integrins. Thus, we hypothesized that other potential candidates from the integrin signalling pathway may be involved in the osteogenic potential of nanotopography. In this context, the aim of this study was to compare the expression of integrin family members of cells grown on nanotopography with machined surface. Machined Ti discs ...

Activins belong to the transforming growth factor-β superfamily, and they regulate bone formation by controlling both osteoclast and osteoblast behaviour. We have previously shown that activin-A strongly inhibited matrix mineralization in osteoblast cultures, and that activin A-signalling was most effective before the onset of mineralization.The aim of this study was therefore to investigate how an early activin-A pulse affected osteoblast mineraliz...

Osteoclasts resorb bone matrix through a specific adhesion structure called the sealing zone or actin ring, which is based on a belt of podosome. Much remains to be uncovered regarding the molecular mechanisms driving podosome organization into superstructures in particular the osteoclast podosome belt. Proteomic analyses in osteoclasts revealed the adaptor protein tensin 3 as a partner of Dock5, a Rac exchange factor necessary for podosome belt formation and bone resorption. ...