Bone Abstracts

Aim: To assess long term safety of low dose pamidronate therapy and the effects on pubertal growth spurt.Methods: A retrospective study was conducted in 36 girls and 21 boys whose mean age was 4.1±3.6 years at baseline. Intravenous pamidronate 3–4 mg/kg per year once daily therapy with 2–4 cycles/year for 0.5–15 years) was given with physical therapy and orthopedic surgery as appropriate. Mobility score, height, puberty and bone miner...

Osteoporosis pseudoglioma syndrome (OPPGS) is a rare autosomal recessive disorder characterised by congenital or juvenile-onset blindness, severe juvenile-onset osteoporosis, and skeletal fragility. OPPGS is caused by loss-of-function mutations in the LRP5 gene, a member of the LDL receptor family. It activates the canonical Wnt/β-catenin pathway, regulating osteoblastic bone formation. We investigated a 40-year-old Caucasian male presenting with congenital blind...

Objective: We investigated the effect of creatine administered in the diet for 10 weeks on bone turnover and bone quality in male Wistar rats.Methods: Rats were divided (after approval of Ethical committee 9814/2008–30 and 30793/2010–30) into four groups, ten animals in each group. G1 SLD-A group were fed a standard laboratory diet (SLD). G2 SLD-B group were fed SLD and 12 h before rats were sacrificed diet was removed. G3...

Objective: We investigated the effect of arginine administered for 10 weeks in diet on bone metabolism in male Wistar rats.Methods: Rats were divided (after approval of Ethical Committee 9814/2008-30 and 30793/2010-30) into four groups, ten animals in each group: G1 SLD-S group were fed a standard laboratory diet (SLD). G2 SLD-H group were fed SLD and 12 h before rats were sacrificed diet was removed. G3 ARG-S group was fed wit...

Osteoporosis, as a global public health concern, may be induced by various hazardous substances such as cadmium (Cd) and pesticide diazinon (DZN). Selenium (Se), an essential trace element, can adversely modify bone structure in higher concentrations. The current study was designed to investigate structural changes in femoral bone of adult male rats after simultaneous co-administration to Cd, DZN, and Se. A total of 20 male Wistar rats were randomly divided into two experiment...

Introduction: Granulocyte colony-stimulating factor (G-CSF) is widely used to mobilize peripheral blood stem cells (PBSC) and enable PBSC collection by apheresis. Although bone pain is a common adverse event following G-CSF treatment, little is known on its effect on bone metabolism.Methods: Markers of bone turnover (OC, osteocalcin, β-CTx, bALP, C-terminal telopeptide of type I collagen, bone specific alkaline phosphatase, TRAP, tartrate resistant ...

MicroRNAs are important regulators of gene expression with documented role in bone metabolism and osteoporosis. Moreover, the use of miRNAs constitutes potential therapeutic targets. Our aim was to identify miRNAs differentially expressed in fractured compared to healthy bone. Additionally, we performed a miRNA profiling of primary osteoblasts to assess the origin of the differentially expressed miRNAs. Total RNA was extracted from fresh femoral neck trabecular bone from women...

Aims: To investigate the role of interleukin-6 (IL-6) as a modulator of osteoclastic resorption in vitro in acute Charcot osteoarthropathy.Material and methods: Peripheral blood mononuclear cells were isolated from six patients with acute Charcot osteoarthropathy, five diabetic and five healthy controls and cultured in vitro on bovine bone disks for 21 days in the presence of i) macrophage-colony stimulating factor (M-CSF) and receptor ...

Osteoporosis is a common disorder with a partly genetic pathogenesis. Interaction between RANKL and its receptor RANK is essential in bone remodeling.We therefore investigated the effect of polymorphisms in the RANK and RANKL genes and interaction between the effects on bone mineral density (BMD) and vertebral fractures.The study was a case-control study with 462 osteoporotic patients and 336 controls. Ten polymor...

Background: Bone marrow (BM) adiposity is inversely related to bone mineral density and increases with ageing and menopause. We previously observed that the variation in BM fat fraction was more pronounced in premenopausal women compared to men and postmenopausal women. We hypothesized that the variation in BM fat fraction in premenopausal women is associated with hormonal variations during the menstrual cycle.Objective: To investigate the dynamic change...