Bone Abstracts

see PP245....

Osteocytes differentiate from osteoblasts, are embedded in mineralised matrix and are critical regulators of bone remodelling. In vitro osteocyte models are limited to cell lines in monolayer, which do not represent their 3D environment in vivo. We have shown that osteoblasts in 3D gels differentiate along the osteocytic pathway. Since IGF1 regulates osteoblasts, and is involved in osteocyte response to mechanical loading, we hypothesised that IGF1 modulates ...

Geroderma osteodysplasticum (GO) is a rare autosomal recessive connective tissue disorder characterised by progeria like facies, wrinkled lax skin, joint hypermobility, congenital dislocation of hips and propensity to fragility fractures. In the past 25 years, five patients (three females and two males) diagnosed with GO were referred to our Paediatric metabolic bone service for assessment and management of secondary bone problems. All five children were born to consanguineous...

Destruction of the articular cartilage is the major feature of Osteoarthritis (OA). Ageing is the primary risk factor, but how ageing results in OA is still an enigma. In OA, articular chondrocytes degrade their own matrix, while in healthy articular cartilage they preserve it.Transforming growth factor β (TGFβ) is a central regulator of chondrocyte proliferation, differentiation and extracellular matrix production. Deregulation of TGFβ si...

Objectives: Glucagon-like peptide 1 is secreted by intestinal L-cells into the blood supply in response to nutrients in the intestine. Although osteoblasts express the GLP-1 receptor (GLP-1R), the main action of the GLP-1/GLP-1R pathway in bone physiology and bone quality is unknown. The aim of the present study was to investigate bone strength and quality in a mouse model of GLP-1R deficiency.Materials/methods: Eight 16 weeks-old GLP-1R knock-out male m...

see PP381....

The problem of early diagnosis of rheumatoid arthritis remains an important area of research in rheumatology. We investigated changes in bone metabolism, bone mineral density in early rheumatoid arthritis (ERA; up to 12 months). Data were compared with changes in the MRI study of the dominant hand.We observed 24 patients with ERA, the average age – 33.6±5.7 years. The men were 6 (25%), women – 18 (75%). Bone mineral density (BMD) was deter...

We have reported femoral osteopenia in short term-vitamin D restricted rats without deterioration in tibial cortical bone volume (CBV), geometry or strength1. This study aimed to establish the effect of extended vitamin D deficiency in aged rat tibial volume and strength. Female Sprague-Dawley rats (9 m, n=6/group) were fed a diet containing varying vitamin D3 (D) levels (0, 2, 12, and 20 IU/day) with either low (0.1%, LCa) or high (1%, HCa) diet...

Objectives: Glucose-dependent insulinotropic polypeptide (GIP) is secreted by intestinal K-cells into the blood supply in response to nutrient ingestion and absorption. Although osteoblasts and osteoclasts express the GIP receptor (GIPR), the main action of the GIP/GIPR pathway in bone physiology and bone quality is unknown. The aim of the present study was to investigate bone quality in a mouse model of GIPR deficiency.Materials/methods: Eleven 16 weeks...

For a reliable assessment of bone’s material quality in a clinical environment, a fast way to measure the mechanical properties of bone is needed. The investigation of material heterogeneity and anisotropy resulting from bone remodeling and mineralization requires an imaging technique with micrometer resolution. Scanning acoustic microscopy (SAM) using high-frequency lenses allows measuring the stiffness of bone under wet conditions in a non-destructive way with this spat...