Bone Abstracts

Adult mesenchymal stem cells (MSCs) are of interest in the fields of cell therapy and tissue engineering thanks to their potential of differentiating into distinct cell lineages, e.g. osteoblast, chondrocyte, myoblast, and adipocyte. As the capacity of differentiation may vary according to the cell source, here, we compared the potential of osteoblast differentiation of MSCs derived from either bone marrow or adipose tissue. MSCs from rat bone marrow and adipose tissue were cu...

Polyphosphates (polyPs) are inorganic phosphate chains found in many cell types with higher concentrations in bone cells. As a source of inorganic phosphate (Pi) and an effective calcium reservoir due to chelation, PolyPs enable total Ca2+ and PO42− concentrations above those required for apatite saturation. Alkaline phosphatase (ALP) cleaves Pi from polyP, thus polyPs may be involved in apatite mineralization.Aim: ...

Wnt signaling is a major regulator of bone metabolism. We recently reported that mutations in WNT1 gene in humans cause early onset osteoporosis and severe osteogenesis imperfecta. To identify the cellular source and the mechanisms causing these severe phenotypes we generated and analyzed global and conditional Wnt1 knockout mice.Heterozygous Wnt1+/− mice were viable and fertile but Wnt1−/− embryos were lost in ute...

Marine cyanobacteria have been recognized as an important source of bioactive compounds. The cytotoxicity on cancer cell lines has been extensively explored and several cyanobacteria metabolites are already described as potential anticancer compounds or are considered useful templates for the design of new anticancer drugs. The majority of compounds have been isolated from filamentous or colonial cyanobacteria that growth in high densities along shores. In contrast, picoplankt...

This study is focused on the effects of the synergic use of vitamins D and K on human osteoblasts primary cultures derived from osteoporotic and normal patients. The aim of this work is the evaluation of the different cellular behaviour in response to the lipophilic vitamins stimulation. We included 20 osteoporotic and 20 control patients in age between 35 and 50 and in age between 55 and 85. All patients gave informed consent for using bone samples as a source of bone cells. ...

All biological tissues, including bone, require vascular support to survive. In fact, bone is a highly vascularized tissue, although this aspect of bone is often overlooked. Extensive work has demonstrated that the blood vessels in bone are necessary for nearly all skeletal functions, including development, homeostasis, and repair. In addition, blood vessels lost due to trauma regenerate, and new bone tissue formed in response to injury is aggressively vascularized. As a conse...

Numerous studies have reported beneficial effects of multipotent mesenchymal stem cells (MSCs) in tissue repair and regeneration. These multipotent cells can be isolated from many different adult tissues and give rise to different cell lineages. The most well-characterized source for adult stem cells is still adult bone marrow, however in the past decade, subpopulations of stem cells have been isolated from dental tissues. Dental pulp has been identified as a promising source ...

Objective: We aimed to investigate associations between early-life vitamin D status, mode of infant milk-feeding and bone outcomes at five years.Methods: Participants were from the prospective mother-infant SCOPE-BASELINE Birth Cohort Study. Serum 25 hydroxyvitamin D (25(OH)D) concentrations were quantified at 15 weeks gestation, in umbilical cord sera and at two and five years using a gold-standard CDC-accredited LCMS method. Whole-body bone mineral con...

Despite widely accepted classical mechanical theory of buttresses as a load bearing areas in the midfacial skeleton, the data related to its microstructural adaptation on functional demands are scarce. In this study we investigated microstructural features of the different regions in the facial bones in relation to the occlusal load dissipation using a combination of finite element analysis (FEA) and micro-CT analysis (μCT). The FEA was performed on the model of the dry h...

The clinical orthopaedic problem of delayed healing or non-union after complex fractures affects 5–10% of all patients, especially within the elderly population. Recently several in vitro studies showed that CD133+ cells bare angiogenic capacities and contribute to a better outcome concerning ischemia induced angiogenesis in vivo. A local administration of these specific cells to the fracture gap appears feasible as a new treatment option for biological ...