Bone Abstracts

Histomorphometry is often adopted as a methodological approach in research of femoral bone structure. However, it is common do not describe technical details of procedure especially on the steps of macroscopy, paraffin embedding and microtomy. So we propose a simplified, reliable and reproducible method of histological processing for intertrochanteric region and femoral neck of mice, to ensure the achievement of tissue sections with similar structures, cell populations and his...

Introduction: Osteocytes play a major role in the bone remodelling unit (BRU). Thus, we hypothesize that mitochondrial bioenergetics impairment and mitochondrial/peroxisomal fatty acid β-oxidation unbalance is a cause of osteocytes metabolic decline during 17β-estradiol (E2) reduction. E2 and a phytochemical substitute, coumestrol (COU) were used (30 mg/kg during 24 h in ovariectomized rats in order to compare bone loss with sham-operated animal...

Recent studies suggest that the concentration and genotype of vitamin D binding protein are important factors that determine the bioavailability of 25OH Vit-D in blood. It has been suggested that measurement of free, non-protein bound 25OH Vit-D in serum, may provide more relevant diagnostic information than total 25OH Vit-D, for instance in chronic kidney disease, bladder cancer and pancreatic cancer, or in hemodialysis patients.To measure free 25OH Vit...

Introduction: Paget’s bone disease (PBD) is the second most prevalent metabolic bone disease. Most patients present with pain or fracture but many remain asymptomatic. Evidence suggests a significant reduction both in its prevalence and clinical severity. Recent papers described differences in clinical course and therapeutic options in the last 10–15 years.Objective: To characterize PBD differences between patients having been diagnosed before ...

Background: Higher BMI values are associated with greater bone mineral density (BMD) resulting in a site-specific protective effect for fragility fractures. However, pathophysiologic influence of central adiposity on bone quality may oppose the seemingly positive influence of a greater mechanical loading with higher body masses. The objective of our study was to evaluate the impact of visceral fat on trabecular bone microarchitecture in postmenopausal obese women.<p class=...

Background: Zoledronate, a third generation bisphosphonate, has showed high efficacy in the inhibition of bone resorption. The objective of this observational study was to assess short and long-term efficacy and safety of zoledronate in the treatment of active Paget’s disease (PD).Methods: Patients with active PD treated with zoledronate 5 mg were consecutively recruited. Clinical and laboratory parameters were determined before, at 3 and every 6 mo...

Young transsexuals at a minimum age of 12 years are treated with GnRH-analogues (GnRHa) to suspend pubertal development until the addition of hormones of the desired sex is started at a minimum age of 16 years. The effect of this treatment on adult bone mineral density (BMD) is still unknown. We aimed to assess BMD at the age of 22, as this is near its peak in healthy individuals.In this prospective observational study 19 female to male (FtM) and 16 male...

Purpose: Osteoclastogenesis is enhanced in osteoarthritis (OA). We have demonstrated that cartilage breakdown is reduced when osteoclastogenesis is inhibited in murine models. Wnt activity, known to regulate the bone and chondrocyte cell function, might contribute to the mechanisms that promote cartilage catabolism. Our purpose was to evaluate whether osteoclast-secreted molecules affect the chondrocyte metabolism and assess the contribution of Wnt pathway.<p class="abstex...

There is the question, if routine screening of hypercalcemia and hypovitaminosis D in the whole Czech population may be considered meaningful and economical.Primary hyperparathyroidism is a frequent cause of hypercalcemia. At our clinic we surgically treat 180 patients diagnosed for primary hyperparathyroidism on average per year. In the sample we detect 24% rate of ectopic gland localization. Such ectopic localization complicates effective surgery, stre...

Introduction: One third of patients in the long-term heparin therapy show reduction in bone density. We have shown that heparin binds to bone morphogenetic protein 6 (BMP6) and inhibits its osteogenic activity in vitro. Here we explored whether heparin effects BMP6 mediated bone efficacy in vivo.Methods: We have used a mouse model of postmenopausal osteoporosis and tested the effect of heparin on BMP6 therapy and its osteogenic activity...