Bone Abstracts

The cathepsin K inhibitor odanacatib (ODN) is a bone formation-sparing inhibitor of osteoclastic resorption activity. This drug is currently under development for the treatment of postmenopausal osteoporosis. To support the bone safety profile of ODN, we evaluated the effects of ODN on trabecular bone hard tissue properties in the estrogen-deficient model of the ovariectomized (OVX) rhesus monkeys. Animals (n=16/group, age 11–22 years) were treated immediately af...

The purpose of this study was evaluated the osteogenic differentiation in vitro of mesenchymal stem cells (MSC) of SHR (Spontaneously Hypertensive Rats) and Wistar (Normotensive) rats.Therefore, MSC from femoral bone marrow of Wistar and SHR rats 4 weeks old were utilized. The experimental protocols were approved by the Animal Experiments Local Ethics Committee (Process 00716-2012). The osteogenic medium (OM) consist of the proliferation medium (MEM) supplemented with...

Titanium implants have been extensively used in dentistry as a replacement of absent dental elements. The biocompatibility of a material depends on cellular response in contact with a surface. The microarray technology is a tool to obtain an overview of the cell state in terms of large-scale transcriptional expression in different situations. Thus, the objective of this investigation was to evaluate the transcriptional profile of osteoblastic cells from human alveolar bone cul...

Background: Vitamin D (Vit-D) plays a role in calcium metabolism during pregnancy, and studies have found widespread deficiency in this period. Its significance is still not fully understood, although low Vit-D levels have been associated with increased risk for preeclampsia, bacterial vaginosis, neonatal sepsis and early respiratory infections in the infant. Supplementation to the pregnant mother remains a matter of debate; thus, it is important to better understand the cause...

ADO2 is a debilitating genetic bone disease causing multiple fractures and other severe symptoms. A mouse model of ADO2, harbouring the heterozygous Clcn7G213R gene mutation, phenocopies the human syndrome. The Clc7 gene encodes the ClC7 dimeric 2Cl−/1H+ antiporter that is almost ubiquitously expressed, although the mutations hit especially the osteoclasts impairing bone resorption. By immunofluorescence, we observed that the mutant ClC7 ...

We previously demonstrated the involvement of Lcn2 in bone loss induced by mechanical unloading. This prompted us to investigate bone phenotype of Lcn2−/− mice by μCT, which showed an osteopenic phenotype, characterized by 40% lower trabecular bone volume, 50 and 21% lesser trabecular number and thickness, respectively, and 20% higher trabecular separation, compared to WT, while cortical thickness was significantly lower (40%) only in elderly Lcn2<s...

Dominant osteogenesis imperfecta (OI) is a bone disease mainly caused by collagen type I mutations and characterized by bone fragility and growth delay. Nowadays no definitive cure is available. A zebrafish OI model (Chihuahua) carrying an heterozygous G574D substitution in the α1 chain of collagen type I was generated by ENU mutagenesis and is available in our laboratory. Control (WT) and mutant (Chi+/−) fish growth was followed up from day 1 post fertilization to ...

After many years of study, researchers show that obesity can harm the bones. It is not known whether the increased fat mass can accelerate the process of bone loss. The aim of this study was to evaluate whether high-fat diet accelerates the process of bone loss in ovariectomized rats. This study was approved by the Ethics Committee of our Institution (188/2013). 40 female wistars rats with body mass of 60 g were used in this study. They were equally divided into four experimen...

In recent years, latex membrane synthesized from natural latex extracted from rubber tree Hevea brasiliensis, has been studied as an important biomaterial to assist in tissue regeneration process, since it has ability to induce neovascularization and tissue proliferation, and is a low-cost and easy material handling. The aim of this study was to evaluate mechanical properties of bones after treatment with natural latex membrane in bone defects created in the tibiae of...

Introduction: Osteopenia and osteoporosis are osteometabolics disorders characterized by decreased bone mineral density (BMD) with deterioration of bone microarchitecture. Since the beginning of the space age, there was a growing interest in trying to reproduce on Earth an environment that could simulate the effects of microgravity to induce bone, a state of osteopenia. The aim of this study was to present a new osteopenia induction model through the full body suspension.<...