Bone Abstracts

Background: In patients with gout, urate deposition has been observed both adjacent to and within tendons, suggesting that monosodium urate monohydrate (MSU) crystals are likely to be in direct contact with tenocytes, the stromal cells of tendons. The aim of this study was to determine the effects of MSU crystals on tenocyte viability and function.Methods: Cultures of primary rat tenocytes were prepared from Wistar rat tails. Primary human tenocytes were...

Objective: Total body less head (TBLH) and spine are the recommended DXA sites for bone health assessment in children and adolescents. However, inter-machine differences will affect use and interpretation of results in clinical care and research applications. We examined BMC and areal-BMD (aBMD) at 4 skeletal sites among healthy children to identify the magnitude of inter-machine differences in Z-scores.Methods: BMDCS evaluated healthy participa...

Objectives: The ketogenic diet (KD) is a medically supervised, high fat, low carbohydrate and restricted protein diet which has been used successfully in patients with refractory epilepsy. Only one published report has explored its effect on the skeleton. We postulated that KD impairs bone mass accrual and examined skeletal health parameters in this patient group.Methods: Patients commenced on the KD from 2002–2009 were enrolled in a prospective, lo...

Sports specific training may have different impact on bone outcomes, and adolescence is a key period to optimize bone health. The aim was to examine hip differences on bone outcomes between osteogenic (football) and non-osteogenic (swimming and cycling) sports in adolescent males.One hundred twenty one males (13.1±0.1 years) volunteered to partake in this study that received ethics approval from the UK National Research Ethics Service. Participants ...

Objectives: Maternal vitamin D status in pregnancy is associated with neonatal bone mass, and altered DNA methylation. Mice exposed to early life vitamin D deficiency have lower bone mass and reduced bone accrual in response to mechanical loading. Using tibias from these mice we assessed DNA methylation of promoters of genetic loci important for bone growth and development.Methods: C57/BL6 mice received a vitamin D replete or deplete diet for 6 weeks per...

Objectives: Low birthweight has been shown to be associated with poorer musculoskeletal health in later life in a variety of epidemiological studies. We investigated relationships between birthweight, and grip strength or magnetic resonance imaging (MRI) measures of muscle volume in UK Biobank.Methods: UK Biobank is a large prospective cohort of men and women aged 40–69 years, including a detailed baseline assessment in which birthweight was collect...

Objectives: To examine the usefulness of Lego® as a visual reinforcer to explain genetic mutations to parents and carers of children and young people who have osteogenesis imperfecta (OI).Methods: Before entering a dedicated OI clinic, patients and carers completed a quantitative questionnaire devised by one of the team (MR), asking how much they knew about the genetic mutations causing OI within their families and whether they wished for a more det...

Introduction: Fracture risk is greater for adults with type 2 diabetes (T2D), despite normal or higher areal bone mineral density (aBMD) compared to controls. Tissue mineral density (TMD), measured by microCT, is more representative of actual mineral density than in vivo aBMD. The aim of this study was to determine whether TMD is greater in adults with T2D, and to investigate the correlates of TMD in adults with T2D.Methods: Using proximal femur...

Objectives: Creation of reference curves for areal bone mineral density (aBMD) and bone mineral content (BMC) with consideration of relevant anthropometric variables.Methods: Analysis of the dual-energy X-ray absorptiometry (DXA) data collected as part of the Bone Mineral Density in Childhood Study1, including 2012 boys and girls, 5–22 years old, with a total of 10 525 visits, resulting in aBMD and BMC observations at the lumbar spine, hi...

Objectives: The determinants of areal bone mineral density (aBMD) and hip geometry estimates in adolescent athletes are poorly understood. This study aimed to identify the determinants of aBMD and hip geometry estimates in adolescent male athletes.Methods: One hundred twenty one males (13.1±0.1 years) were measured: 41 swimmers, 37 footballers, 29 cyclists and 14 controls. Dual energy X-ray absorptiometry (DXA) measured aBMD at lumbar spine, femoral...