Bone Abstracts

Introduction: For the very first time were assessed in vivo the metabolic and lipid profiles of osteocytes. During menopause period the appearance of an osteoporotic condition can be associated with an overall metabolic decline in bone cells, as well an increase of reactive oxygen species (ROS), and we hypothesized that it is mainly attributed to osteocytes metabolic and lipid changes, which could be apparently attenuated after raising blood estradiol (E2) levels. To ...

IκB Kinase β (IKKβ), a key component of NFκB signaling, plays an important role in bone disease and cancer. Genetic and pharmacological inhibition of IKKβ/NFκB signaling increase bone mass and protect against ovariectomy- and inflammation-induced bone loss. Here, we describe a previously unknown role of the IKKβ/FoxO3a axis in bone metastasis associated with breast cancer. We observed that IKKβ expression is prevalent in invasive breast ...

Postmenopausal women with early stage hormone-receptor-positive breast cancer (EBC) are standardly treated with aromatase inhibitors (AIs). However, one side-effect of AIs treatment is a decrease in bone mineral density (BMD) and an increased risk of fracture. The objectives of this study were to examine: i) changes in bone formation (N-terminal propeptide of type I procollagen; PINP) and bone resorption (cross-linked C-telopeptides of bone type I collagen; CTX) markers, as we...

Children with 21-hydroxylase deficiency (21-OHD) need chronic glucocorticoid (cGC) therapy to replace congenital deficit of cortisol synthesis, and this therapy is the most frequent and severe form of drug-induced osteoporosis. In the study we enrolled 18 patients (9 females) and 18 sex- and age-matched controls. We found in 21-OHD patients high serum and leukocyte levels of dickkopf-1 (DKK1), a secreted antagonist of the Wnt/β-catenin signaling pathway, known to be a key...

Patients with type 2 diabetes (T2DM) have an increased risk of hip and vertebral fractures compared to non-T2DM subjects. The bone mineral density (BMD) does not reflect the impaired bone quality in T2DM patients. The aim of the present study was to investigate the relationship between BMD and bone formation (N-terminal propeptide of type I procollagen, PINP; osteocalcin, OC) and bone resorption (cross-linked C-telopeptides of bone type I collagen, CTX) markers, as well as cha...

see PP290....

The effects of prolactin on bone metabolism have been the subjects of several studies. It is believed that prolactin acts directly influencing the synthesis of bone matrix by stimulating the osteoblastic activity, since receptors for this hormone have been identified in osteoblasts and human mesenchymal stem cells (MSCs). However, no study on the effects of prolactin on the osteogenic differentiation of MSCs was found in the literature. The objective of this study was to verif...

Introduction: Osteocytes play a major role in the bone remodelling unit (BRU). Thus, we hypothesize that mitochondrial bioenergetics impairment and mitochondrial/peroxisomal fatty acid β-oxidation unbalance is a cause of osteocytes metabolic decline during 17β-estradiol (E2) reduction. E2 and a phytochemical substitute, coumestrol (COU) were used (30 mg/kg during 24 h in ovariectomized rats in order to compare bone loss with sham-operated animal...

Caffeine is a methylxanthine found in many foods and is widely consumed by the human population. Therefore, its effects and mechanisms in various tissues have been widely studied. Various bone abnormalities have been observed in fetuses from rats treated with caffeine. But the genesis of these abnormalities is not known. The objective of this study was to verify the metabolism and activity of calvaria osteoblasts from offspring of rats treated with caffeine during pregnancy. T...

Thalassemia is a chronic anemia associate with high rates of osteoporosis. To determine how erythropoiesis leads to bone loss in thalassemia, we used the th3/+ thalassemia mouse to study the role of erythropoietin (EPO) and hematopoietic progenitors (HP), since they are both increased in thalassemia.Methods: Bone marrow mesenchymal stem cells (MSCs) cultures and MSC cocultures with various HP from wild type (wt) and th3/+ mice were diff...