Searchable abstracts of presentations at key conferences on calcified tissues

ba0005p145 | Cell biology: osteoblasts and bone formation | ECTS2016

Evaluation of release kinetics and mitogenic capacity of collagen barrier membranes supplemented with the secretome of activated platelets

Mozgan Eva-Maria , Edelmayer Michael , Janjić Klara , Pensch Manuela , Fischer Michael , Agis Hermann

Success in periodontal regeneration through guided tissue regeneration relies on the healing capacity of the host tissue. Platelet preparations are mitogenic and stimulate regeneration through high growth factor levels. Here we assessed collagen barrier membranes (CBM) as carriers for the secretome of activated platelets. We evaluated cell-attachment, the release of the secretome, and cell proliferation.Secretome of washed platelets (washed PSEC) and unw...

ba0003pp375 | Other diseases of bone and mineral metabolism | ECTS2014

Osteoprotegerin and bone-like vascular calcification are predictive markers of vulnerable carotid plaques

Heymann Marie Francoise , Davaine Jean Michel , Laperine Olivier , Merlini Thierry , Guyomarch Beatrice , Chatelais Mathias , Guilbaud Florian , Brennan Meadhbh Aine , Heymann Dominique , Goueffic Yann

Vascular calcification has a marked impact on arterial heterogeneity and plaque stability. Based on a cohort of 116 patients (carotid and femoral endarterectomies), the aim of the present study was to determine the influence of the bone-like vascular calcification and of osteoprotegerin (OPG) on plaque stability.Seventy-three carotid and 43 femoral plaques along with plasma were harvested and analyzed in a single center study. The presence of osteoid met...

ba0001pp8 | Clinical case posters | ECTS2013

Phenotypic change in a patient with hypophosphatasia with the onset of renal failure

Cundy Tim , Michigami Toshimi , Tachikawa Kanako , Dray Michael , Collins John

Hypophosphatasia is a recessively inherited disorder with a wide phenotypic manifestation ranging from lethality in neonates to asymptomatic in adults. The severity of the phenotype is largely determined by the nature of the ALPL mutations. We describe a previously asymptomatic adult whose phenotype dramatically changed after he developed renal failure. A 50-year-old man was diagnosed with IgA nephropathy. At age 52 (eGFR 50 ml/min) he suffered his first metatarsal fr...

ba0003pp162 | Cell biology: osteoclasts and bone resorption | ECTS2014

The role of IL-6 as a modulator of osteoclastic resorption in vitro in acute Charcot osteoarthropathy

Petrova Nina , Petrov Peter , Edmonds Michael , Shanahan Catherine

Aims: To investigate the role of interleukin-6 (IL-6) as a modulator of osteoclastic resorption in vitro in acute Charcot osteoarthropathy.Material and methods: Peripheral blood mononuclear cells were isolated from six patients with acute Charcot osteoarthropathy, five diabetic and five healthy controls and cultured in vitro on bovine bone disks for 21 days in the presence of i) macrophage-colony stimulating factor (M-CSF) and receptor ...

ba0003pp358 | Osteoporosis: treatment | ECTS2014

Zoledronate reverses bone marrow adiposity in disuse osteopenic rats

Jensen Michael Vinkel , Bruel Annemarie , Thomsen Jesper Skovhus

Objective: I.m. injections with Botox (BTX) leads to a paralysis of the muscles, resulting in a rapid loss of muscle and bone mass (disuse osteoporosis). Adipocytes and osteoblasts derive from the same mesenchymal stem cell, and commitment to one linage represses commitment to the other. Furthermore, the existence of a close communication between bone cells and endothelial cells is widely accepted.The purpose of the study was to quantify the changes in a...

ba0004p70 | (1) | ICCBH2015

Ultrasonographic assessment of the skeletal development of the proximal tibia epiphysis, the proximal femur and the distal femur epiphysis in premature and mature newborns

Windschall Daniel , Pommerenke Michael , Rimsl Theresa , Haase Roland

Backround: Usually, skeletal development and bone age in children are still examined by x-ray. In obstetrics the use of fetal ultrasonography (US) of the distal femoral epiphyseal (DFE) and proximal tibial epiphyseal (PTE) ossification centers helps to identify intrauterine growth retardation. And US has been shown to be an excellent tool to assess the mineralization of bones. First ultrasound studies were following the mineralization of bones in young infants.<p class="ab...

ba0005p475 | Paediatric bone disease | ECTS2016

Validation of a novel scoring system, the radiographic global impression of change (RGI-C) scale, for assessing skeletal manifestations of hypophosphatasia in infants and children

Whyte Michael , Fujita Kenji , Moseley Scott , Thompson David , McAlister William

Hypophosphatasia (HPP) is the rare inherited metabolic disease caused by loss-of-function mutations in the tissue-nonspecific alkaline phosphatase (TNSALP) gene. TNSALP deficiency leads to extracellular excess of inorganic pyrophosphate, a bone mineralization inhibitor. Here, we report the validity and reproducibility of a novel scale to quantify HPP-specific radiographic changes in pediatric patients.The Radiographic Global Impression of Change (RGI-C) ...

ba0005lb6 | (1) | ECTS2016

Sclerostin blockade and zoledronic acid improve bone mass and strength in mice with exogenous hyperthyroidism

Tsourdi Elena , Lademann Franziska , Ominsky Michael , Hofbauer Lorenz , Rauner Martina

Hyperthyroidism in mice is associated with a low bone mass, an increased bone turnover and high serum levels of sclerostin, a potent Wnt inhibitor. Here, we explored the effects of either reducing bone turnover with bisphosphonates or increasing bone formation with neutralizing sclerostin antibodies (Scl-Ab) on bone mass and estimated strength in hyperthyroid mice.Twelve-week-old C57BL/6 male mice were rendered hyperthyroid by adding L-thy...