Bone Abstracts

Introduction: Recessive osteogenesis imperfecta (OI) is caused by mutations in genes encoding proteins involved in post-translational interactions with type I collagen. Types VII–IX OI involve defects in the collagen prolyl 3-hydroxylation complex, which modifies α1(I)Pro986. PPIB encodes CyPB, a complex component with PPIase activity and the major isomerase facilitating collagen folding. We investigated the role of CyPB in collagen post-translational modifications a...

Reduced availability of oxygen, i.e. hypoxia, could occur during disuse, bone development, and fracture. Cartilage tissue is avascular in nature and the microenvironment of cartilage is hypoxic. Hypoxic regulation of gene expression generally involves activation of the hypoxia-inducible factor (HIF) transcription pathway. Receptor activator of nuclear factor-kappaB ligand (RANKL) is an osteoblast/stromal cell derived essential factor for osteoclastogenesis. Hypoxia-induced enh...

Cam femoro-acetabular impingement (FAI) surgery restores hip range of motion by trimming the head–neck junction, which weakens the proximal femur. Sheep femurs resemble cam deformity and might be suitable for evaluating the impact of the surgical correction. Accordingly, this study addressed two questions: How does increasing head-neck resection affect ovine femoral strength? Are quantitative computed tomography (QCT)-based FE models able to discriminate the observed chan...

Background: Recurrent abdominal pain is common in children and adolescents. This symptom could be raised by the complex of both functional and organic etiologies.Presenting problem: We put forward a seventeen-years-old adolescent girl with personal history of repeated abdominal pain and urinary tract infections. Her family history was negative.Clinical management: Our patient was admitted to hospital due to recurrent abdominal pain...

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Introduction: Calcification of vessels, especially media calcification, in combination with bone demineralization and disturbed bone metabolism is abundant in patients suffering from end stage renal disease (ESRD). In this project, we compare biomarkers of calcification with a focus on microRNAs from ESRD patients listed for renal transplantation (RTX) and healthy controls.Methods: Samples are collected from kidney transplant patients. At the same time t...

Subject: To examine the relationship between calcification of the coronary arteries, coronary atherosclerosis and indicators of bone mineral density (BMD) among males with coronary heart disease (CHD).Materials and methods: Seventy-four males with documented CHD were examined. BMD was assessed by dual energy absorptiometry with T-criterion definition of the proximal femur and lumbar spine, coronarography, multispiral computed tomography with quantificati...

Glucocorticoids (GC) are effective drugs in the treatment of inflammatory diseases, including various forms of arthritis. However, their use is limited by negative effects on bone mass and strength, resulting in increased osteoporotic fractures. Conditional knockout mice demonstrated that the GR in osteoblasts is essential for GC-dependent bone loss. Recent studies show that GC profoundly inhibit Wnt signaling by stimulating the expression of Wnt antagonists such as dickkopf-1...

Objectives: Dysregulation of systemic phosphate homeostasis is often associated with impairment of musculoskeletal tissue function. Many factors such as calcium levels and dysegulated endocrine mechanisms are thought to contribute. Inorganic phosphate and FGF23 have been shown to act via similar signaling pathways in several cell types but we are not aware of any detailed investigations into their effect on the differentiation and viability of skeletal muscle cells. W...

Background: Disturbance of systemic phosphate homeostasis is often associated with musculoskeletal dysfunction. Multiple factors related to the underlying condition such as calcium levels and endocrine mechanisms are thought to contribute. Distinct effects of inorganic phosphate itself as well as its main regulator FGF23 by activation of similar pathways have been shown in several cell types. We are not aware of any detailed investigations into their effect on the differentiat...