Bone Abstracts

Tannerella forsythia is a gram-negative, anaerobic, asaccharolytic, fusiform bacterium. The presence of the bacterium is associated with various forms of periodontal disease, including gingivitis, chronic and aggressive periodontitis. The expression of GroEL, a bacterial heat shock protein, increases in stressful conditions such as infection. GroEL is a moonlighting protein that has multiple functions. The aim of this study was to evaluate the effect of T. forsyth...

Osteoclasts are bone-resorbing multinucleated cells that differentiate from monocyte/macrophage-lineage precursors. Bone destruction and osteoporosis are attributed to excessively activated osteoclasts. Osteoclast differentiation is governed by diverse regulatory processes including nuclear factor-activated T cells c1 (NFATc1) activation in response to RANKL. The mechanism of epigenetic regulation of NFATc1 in osteoclastogenesis not investigated yet. Here we test a hypothesis ...

Pseudovitamin D-deficiency rickets (PDDR) is a rare autosomal recessive disorder resulting from a defect in renal 25-hydroxyvitamin D 1α-hydroxylase, which is encoded by the CYP27B1 gene. To our best knowledge, 48 mutations of the CYP27B1 gene have been identified so far. In the present study, we investigated CYP27B1 mutations in seven individuals from six separate families and identified nine different mutations: two novel missense mutations (G194R, R259L), three novel a...

Histomorphometry is often adopted as a methodological approach in research of femoral bone structure. However, it is common do not describe technical details of procedure especially on the steps of macroscopy, paraffin embedding and microtomy. So we propose a simplified, reliable and reproducible method of histological processing for intertrochanteric region and femoral neck of mice, to ensure the achievement of tissue sections with similar structures, cell populations and his...

Arterial calcification is now considered as an actively regulated process resembling osteogenesis orchestrated by a number of systemic or constitutively expressed mediators. Genetic studies of rare inherited syndromes have identified key regulators of arterial calcification. Based on the pathogenic principles causing the diseases these can be classified into three groups:i) Disorders of an increased extracellular inorganic phosphate/inorganic pyrophospha...

Introduction: The evidence for the effectiveness of early rehabilitation of patients with rheumatoid arthritis (RA) is scanty. The aim of the study is to evaluate the efficiency of rehabilitation program for patients with early RA.Methods: 34 study group patients with early RA underwent 6-month rehabilitation (hospital stage: 15-min local air cryotherapy (−;60 °C, Criojet Air C600) for hand, knee or ankle joints, 45-min therapeutic exercises u...

Bisphosphonates are the mainstay of medical therapy in the fracturing child with osteoporosis. The majority of the data in children pertains to i.v. pamidronate use in children and adolescents with osteogenesis imperfecta (OI), where pamidronate has been associated with improvements in bone mineral density, cortical thickness, vertebral shape, pain, mobility and height.1 Side-effects of pamidronate including acute phase response to the initial dose and retardation o...

Background: Stuve–Wiedemann syndrome (SWS, OMIM 601559) is a severe autosomal recessive condition characterized by bowing of the lower limbs with cortical thickening, wide metaphyses, abnormal trabecular pattern and camptodactyly. Additional features include dysautonomia symptoms with temperature instability, respiratory distress and sucking/swallowing difficulties in the first months of life.Most SWS cases do not survive beyond the first y...

Background: Hypophosphatasia (HPP) results from inactivating mutation(s) in the gene for tissue non-specific alkaline phosphatase (TNSALP). Substantial improvement has been reported in skeletal mineralization and physical function in patients (pts) with life-threatening perinatal and infantile HPP treated for 48 weaks with asfotase alfa, a bone-targeted recombinant human TNSALP.Objective: To evaluate long-term efficacy and tolerability of asfotase alfa i...

Background: Eosinophilic granuloma (EG) typically affects children in the first two decades of life, mainly involving vertebrae and long bones. This study presents the first –to the best of our knowledge - case of a unifocal EG of the femur, successfully treated with percutaneous image-guided radiofrequency ablation (RFA), which was monitored pre- and post-ablation with MRI-scans to validate the efficacy of this method.Presenting problem: A 16-year-...