Bone Abstracts

High fat diet (HFD), obesity, and physical inactivity characterize the modern lifestyle. This can lead to coronary heart diseases and type 2 diabetes mellitus. Recent studies have shown that these conditions frequently are associated with poor bone quality. However, the molecular mechanisms are poorly understood. To analyze the effect of HFD and exercise (EX) on bone homeostasis, we fed 6-week-old C57BL/6 mice a high fat (60% fat) or standard chow diet for 10 weeks (10–20...

Clinical data suggest that the mTOR inhibitor everolimus may have bone protective effects in addition to its anti-tumor effects in women with ER+/HER2− metastatic breast cancer receiving hormone-ablative therapies. Based on these findings, we tested the hypothesis, whether everolimus exerts concurrent anti-tumor effects while protecting the skeleton in murine models. Thus, we assessed bone metabolism and anti-tumor effects in osteolytic cancer models upon exposure to eve...

Bone metastasis (BM) is a dismal complication of cancer, occurring frequently in patients with advanced breast carcinoma. During metastatic progression, carcinoma cells harness osteoclast (OC) activity, promoting osteolysis. To adapt to hypoxia and/or to support proliferation, carcinoma cells adopt primarily glycolysis for energy production, therefore releasing lactic acid in the microenvironment through monocarboxylate transporter 4 (MCT4). Stressed by tumor cells, osteoblast...

High fat diet (HFD), obesity, and physical inactivity characterize the modern lifestyle. This can lead to coronary heart diseases and type 2 diabetes mellitus. Recent studies have shown that these conditions frequently are associated with poor bone quality. However, the molecular mechanisms are poorly understood. To analyze the effect of HFD and exercise (EX) on bone homeostasis, we fed 6 week-old C57BL/6 mice a high fat (60% fat) or standard chow diet for 10 weeks (10-20 mice...

The purpose of this study was to analyze the influence of diet supplemented with hydrosoluble soy extract – HSE in bone mass of male and healthy Wistar rats. Sixteen (16) 30 days old animals were kept in controlled conditions with light/dark cicle and fed with commercial food and filtered water. Randomized into two groups: control group received 500 ml of water and 300 g of food, daily; supplemented group S, in addition of water and food, they also received 500 ml of soy ...

Compliance with prescribed drugs is poor in most chronic conditions and osteoporosis is no exception. Compliance integrates the concepts adherence (how much drug is taken) and persistence (for how long) and also if the patient follow the instructions for a correct use of the medication. Between 50 and 75% of patients initiating antiosteoporosis drugs are not taken the treatment 1 year later. Obviously, this problem significantly decreases the effect of drugs. A smaller increas...

Chordomas are rare, low to intermediate grade malignant bone tumors of the axial skeleton. Current treatment options are limited to surgical procedures as chordomas are largely resistant to conventional radiation and chemotherapy. Cell lines are valuable tools to explore molecular mechanisms involved in tumorigenesis and they have a fundamental impact on the development of new anticancer agents. We established a novel chordoma cell-line, MUG-Chor1, from a recurrent morphologic...

The skeleton is a preferred site of metastasis in patients with advanced breast cancer, and bone loss is one of the major complications of breast cancer metastasis. Therefore, prevention of bone metastasis is clinically important. Our previous observation of an anti-osteoclastic activity of Trolox, a vitamin E analogue, led us to investigate whether Trolox could inhibit bone metastasis and bone destruction induced by breast cancer. I.P. administration of Trolox markedly inhibi...

Purpose: To investigate the effect of enamel matrix derivative (Emdogain) on the attachment, growth behavior and the genetic effect of human gingival fibroblasts (HGF) cultured on zirconium disc surfaces.Materials and methods: HGF cells were cultured on i) zirconium discs without enamel matrix derivative (EMD), ii) zirconium discs with EMD 25 μg/ml, and iii) zirconium discs with EMD 100 μg/ml. The cell proliferation activity was evaluated throu...

Background: Osteogenesis imperfecta (OI) is a rare disease characterized by increased fracture rate and bone deformities. Patients are classified by phenotype and most are affected by mutations in COL1A1/2.Patients with OI type V present with specific clinical symptoms including hyperplastic callus formation, only mildly decreased height, metaphyseal lines and a calcification of the membrane interossea of the forearm. The disease causing mutation for OI ...