Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp243 | Cell biology: osteocytes | ECTS2013

Single osteocyte gene expression in an in vivo model for load-induced bone adaptation

Wilson Robin , Trussel Andreas , Webster Duncan , Kurth Felix , Dittrich Petra , Muller Ralph

It is hypothesized that osteocytes regulate bone adaptation by sensing mechanical strains in their microenvironments and signaling net bone formation or resorption. Owing to bone’s anisotropic architecture, individual osteocytes within a bone experience varying strains under mechanical loading. Thus, to accurately determine the relationships between mechanical strain, osteocyte behavior, and bone remodeling, it is crucial to use a single-cell approach. Using an in viv...

ba0001pp318 | Osteoporosis: evaluation and imaging | ECTS2013

Binding kinetics of fluorescent bisphosphonates as a tool for monitoring bone dynamics in vivo

Tower Robert , Campbell Graeme , Muller Marc , Will Olga , Grundmann Frederieka , Schem Christian , Gluer Claus , Tiwari Sanjay

Bone resorption and deposition occur in a tightly regulated fashion reflecting the coupled activities of osteoclasts and osteoblasts. Several pathological conditions perturb this balance between bone synthesis and resorption, including osteoporosis and skeletal metastases. The uncoupling of remodeling activities contributes to disseminated tumor cells homing to the bone and to tumor growth in bone. Therefore, a reliable marker of bone remodeling would be useful to provide a st...

ba0001pp437 | Osteoporosis: treatment | ECTS2013

The spatial relationship between bone formation and bone resorption in healthy and ovariectomized mice treated with PTH, bisphosphonate or mechanical loading

Ruffoni Davide , Weigt Claudia , Fattorini Elisa , Levchuk Alina , Schulte Friederike , Kuhn Gisela , Muller Ralph

Bone is continuously remodeled to remove damage, to adapt to changes in mechanical demands and to regulate calcium homeostasis. The first aim is accomplished by coupled bone formation and resorption whereas adaptation requires sites of formation to differ from those of resorption. The regulation of circulating ions is achieved by a stochastic exchange of bone packets. Here, we investigated these different aspects of remodeling in healthy and ovariectomized (OVX) mice treated w...

ba0003pp143 | Cell biology: osteoblasts and bone formation | ECTS2014

Utilization of L-mimosine in pulp regeneration: lessons from cell culture and tooth slice organ cultures

Muller Heinz-Dieter , Trimmel Katharina , Cvikl Barbara , Gruber Reinhard , Agis Hermann

After trauma or carious lesion dental pulp healing is difficult to predict. In addition systemic diseases like diabetes mellitus can impair the regenerative capacity. New regenerative strategies target prolyl hydroxylase (PHD) by pharmacological inhibitors to stimulate hard and soft tissue healing. PHD inhibitors such as L-mimosine (L-MIM) induce vascular endothelial growth factor (VEGF) production by promoting angiogenesis. However, it is unclear if L-MIM is a feasible tool t...

ba0003pp225 | Osteoporosis: evaluation and imaging | ECTS2014

Utilizing bisphosphonate binding kinetics and soft tissue-derived input functions to differentiate changes in long bone and vertebra bone metabolism using in vivo fluorescent molecular tomography

Tower Robert J , Muller Marc , Will Olga , Tiwari Sanjay , Gluer Claus C , Campbell Graeme M

Bone resorption and formation occur in a tightly regulated fashion reflecting the coupled activities of osteoclasts and osteoblasts. Several pathological conditions perturb this balance, including osteoporosis and skeletal metastases. In the case of metastases, the uncoupling of resorption and formation activities contributes to disseminated tumor cells homing to the bone and to tumor growth within the bone in highly localized regions. Therefore, a site-specific marker of bone...

ba0004p144 | (1) | ICCBH2015

Hypomagnesaemia due to lead poisoning in the context of a heterozygous CLDN-16 mutation

Ramaswamy Priya , Kurre Malathi , Muller Dominik , Dargan Paul , Gevers Evelien , Allgrove Jeremy

Background: 3 year old boy born to non-consanguineous parents. He was diagnosed to have autism at 2 years of age. He had a history of pica.Presenting problem: He was admitted with severe carpopedal spasms of hands and feet. Investigations revealed severe hypomagnesaemia at 0.26 (0.7–1.0) mmol/l, hypocalcaemia at 1.59 (2.2–2.6) mmol/l, hypokalaemia, hyponatremia and moderately low vitamin D levels. Parathyroid hormone concentration was low. Urin...

ba0005p201 | Cell biology: osteoclasts and bone resorption | ECTS2016

Galectin-1 is involved in osteoclast biology

Muller Josephine , Binsfeld Marilene , Dubois Sophie , Carmeliet Geert , Beguin Yves , Heusschen Roy , Caers Jo

Osteolytic bone disease (OBD) is a frequent complication of multiple cancers, such as multiple myeloma. OBD is due to the disruption of balanced bone remodelling, with higher bone resorption due to increased osteoclast activation and osteoblast inhibition. Lectin–glycoprotein interactions have been implicated in osteoclast formation. In the current study, we set out to identify lectins that are involved in osteoclastogenesis and to study their role in this process. We ant...

ba0006p148 | (1) | ICCBH2017

Muscle density measurement in muscular dystrophy

Bechtold Susanne , Blaschek Astrid , Muller-Felber Wolfgang , Roeb Julia , Weissenbacher Claudia , Sydlik Carmen , Schmidt Heinrich

Objective: Muscular dystrophy is characterized by lower skeletal muscle quality, lower muscle strength and physical performance. The aim of the study was to assess regional muscle density and its correlation with regional muscle area in Duchenne muscular dystrophy (DMD) subjects and able bodied controls.Method: Skeletal muscle pQCT (peripheral quantitative computed tomography) scans at the non-dominant forearm were performed in patients with muscle dystr...

ba0007p222 | (1) | ICCBH2019

Long-term clinical outcome in chronic recurrent multifocal osteomyelitis (CRMO): the Leiden cohort

Ramautar Ashna IE , Hamdy Neveen AT , Hissink-Muller Petra CE , Cate Rebecca ten , Appelman-Dijkstra Natasha M

Objectives: To characterize clinical features and long-term outcome of CRMO, a rare inflammatory bone disease of childhood and adolescence, which forms part of the spectrum of chronic non-bacterial osteomyelitis (CNO).Methods: We studied 33 patients with an established diagnosis of CRMO followed at the Centre for Bone Quality of the Leiden University Medical Center from 1994 to 2018. Demographic and clinical data were collected at presentation and last f...

ba0001pp469 | Other diseases of bone and mineral metabolism | ECTS2013

MEPE-derived ASARM peptide impairs mineralization in tooth models of X-linked hypophosphatemia

Salmon Benjamin , Bardet Claire , Khaddam Mayssam , Baroukh Brigitte , Lesieur Julie , Denmat Dominique Le , Nicoletti Antonino , Poliard Anne , Rowe Peter S , Linglart Agnes , McKee Marc D , Chaussain Catherine

Mutations in the PHEX gene cause X-linked familial hypophosphatemic rickets (XLH) with severe bone (osteomalacia) and tooth abnormalities being the distinguishing features of this disease. The PHEX mutations lead to an increase in ASARM peptides (acidic serine- and aspartate-rich motif) and osteopontin fragments which inhibit bone extracellular matrix mineralization. MEPE-derived ASARM has been shown to accumulate in tooth dentin of patients with XLH where it may impair dentin...