Bone Abstracts

Destructive bone lesions due to osteolytic bone disease (OBD) are a major cause of morbidity and mortality in multiple myeloma (MM) patients and the development of new therapeutic strategies is of great interest. In this study, we assessed the effect of SRC inhibition with saracatinib (AZD0530, AstraZeneca) on the development of MM and its associated OBD. We first determined SRC family kinase expression in the MM microenvironment and found that myeloma cells express SRC at low...

The aim of the study was to evaluate interrelationships between morphometric, densitometric and mechanical properties of mandible in Polish Merino sheep. Males (n=7) were kept to slaughter age of 5 months of life to obtain mandible. After the slaughter, morphological properties of the mandible such as bone weight and length were determined. Using quantitative computed tomography (QCT) technique and Somatom Emotion, Siemens apparatus, volumetric bone mineral density (v...

Background: The traditional Inuit diet in Greenland consists mainly of fish and marine mammals. The marine diet is an important source for vitamin D in Greenland. Vitamin D has anti-inflammatory capacity but markers of inflammation were high in Inuit with a high intake of marine food items. Yet, the effect of vitamin D on inflammation in Inuit remains unsettled.Objectives: To investigate the association between vitamin D and markers of inflammation in a ...

IκB Kinase β (IKKβ), a key component of NFκB signaling, plays an important role in bone disease and cancer. Genetic and pharmacological inhibition of IKKβ/NFκB signaling increase bone mass and protect against ovariectomy- and inflammation-induced bone loss. Here, we describe a previously unknown role of the IKKβ/FoxO3a axis in bone metastasis associated with breast cancer. We observed that IKKβ expression is prevalent in invasive breast ...

The aim of this study was to determine interrelationships between final body weight, bone-specific alkaline phosphatase (BAP), IGF1 and morphological, densitometric and mechanical properties of deciduous mandibular teeth isolated from seven healthy Polish Merino rams. Four incisors (I1–I4) and second premolar (P2) were obtained from 5-month-old sheep. Teeth were cleaned, weighed, and their length was measured. Mean volumetric tooth mineral...

Osteogenesis imperfecta (OI) type VI is caused by recessive null mutations in SERPINF1, encoding pigment epithelium-derived factor (PEDF), an anti-angiogenic secretory glycoprotein. Dominant mutations in IFITM5, encoding BRIL (Bone Restricted Ifitm-Like), a transmembrane protein upregulated in osteoblasts during mineralization, cause either type V OI (c.-14C>T, addition of 5 amino acids on BRIL) or atypical type VI OI (...

Background: Eosinophilic granuloma (EG) typically affects children in the first two decades of life, mainly involving vertebrae and long bones. This study presents the first –to the best of our knowledge - case of a unifocal EG of the femur, successfully treated with percutaneous image-guided radiofrequency ablation (RFA), which was monitored pre- and post-ablation with MRI-scans to validate the efficacy of this method.Presenting problem: A 16-year-...

Introduction: Biopsy is a milestone in the diagnosis and treatment of skeletal lesions. Closed core-needle biopsy under computed tomography (CT) guidance is the gold standard. Open biopsy on the other hand may lead to more secure diagnosis.Objectives: Aim of this retrospective case-series study was the review of all biopsies (closed and open) performed at our department during the last eleven years in children suffering from bone tumours and the evaluati...

Objectives: Prospective case-controlled study assessing whether the incidence of loosening of implants in patients undergoing Total Knee Arthroplasty (TKA) is higher among those with Secondary Hyperparathyroidism (SP) at the time of index operation.Material and methods: Caucasian female patients with knee osteoarthritis who underwent TKA between November 2004 and March 2007 were enrolled. Exclusion criteria: endocrinopathies, rheumatoid or secondary arth...

Introduction: A synthetic prostacyclin IP receptor agonist (ONO-1301) has been reported to induce the production of endogenous HGF and VEGF in fibroblasts by stimulating cAMP production.Materials and methods: In vitro; murine primary osteoblasts and cell line (ST2, MC3T3-E1, C2C12) were treated with BMP-2 (0–100 ng/ml) and ONO-1301 (0–10−6 M). ALP assay and cell proliferation assay were performed. In vivo analysis, Col...