Bone Abstracts

Osteosarcoma, the main malignant primary bone tumor, affects a ‘young’ population composed of children and young adults. Current treatment consists of tumor resection associated with chemotherapy. Unfortunately in many cases, a lack of response to anti-tumor drugs is observed, leading to development of metastases and to the patient’s death. Because TGF-β promotes metastases from many solid epithelial tumors, we investigated the effect of inhibitory Smad7 ov...

Chondrosarcomas are cartilage-forming, poorly vascularized tumors. With an estimated annual incidence of 1 in 200 000, they represent the second malignant primary bone tumor of adults after osteosarcoma. These tumors are resistant to chemotherapy and radiotherapy, surgical excision remaining the only therapeutic option. However, very few cell lines and animal models are available, and the mechanisms behind their chemoresistance remain largely unknown. Our goal was to establish...

Background: High zoledronic acid (ZOL) dose protocol, one of the most potent inhibitors of bone resorption, is currently evaluated in a phase III clinical trial in Europe for the treatment of malignant pediatric primary bone tumors. The impact of such an intensive treatment on the craniofacial skeleton growth is a critical question in the context of patients with actively growing skeleton, in the light of our previous studies evidencing that endochondral bone formation was tra...

In clinical research protocols, HR-pQCT images (XtremCT Scanco, voxel size: 82 (μm3) are carried out to evaluate trabecular and cortical bone changes induced by osteoporosis and treatments. Micro-computed tomography (μ-CT) has become a standard tool for examination of trabecular and cortical bone in 3D.The purpose of this study is to evaluate the accuracy of cortical and trabecular measurements derived from HR-pQCT images with morpho...

Objectives: Lysophosphatidic acid (LPA) is a potent lipid growth factor which possess several G protein-coupled receptors LPA1-6. We have recently demonstrated that LPA1 receptor-invalidated mice display abnormal bone development and osteoporosis, suggesting abnormal endochondral ossification. We have here further studied the growth plates of LPA1 receptor-invalidated mice.Methods: We performed a microscopic and immuno-histochemistry analysis of the femo...

Objectives: Ghrelin is a peptide hormone secreted in the stomach, which stimulates GH release and food intake. It is also known to have an effect on bone metabolism. The ghrelin specific receptor, GHS-R1a, belongs to the G protein-coupled receptors (GPCRs). Its downstream pathway in osteoblasts remains unclear. We attempted to clarify the way by which ghrelin acts on osteoblast differentiation.Methods: We studied two human osteosarcoma cell lines, MG63 a...

Atypical femoral fractures (AFF) of the subtrochanteric region are rare. Bisphosphonates account to a large extent to their occurrence, however AFF also occur without exposure to bone medication. We here assessed the genetic factors associated with AFF among subtrochanteric fractures. Cases of subtrochanteric or femoral shaft fractures were identified through ICD-10 codes in three French academic centers from 2007 to 2010. Medical records were analyzed by two investigators tha...

I. HistoryII. How the bisphosphonates changed the management plan?In babies No need to rod non walking childrenIn toddlers More Upper extremity surgeriesLong-term problems: Risk of delayed non-union:Several measures were introduced to limit the risk (no Bisphosphonates 48 hours pre-op, and 4 months post-op) as well as technical ad...

Objectives: Ghrelin is an orexigenic hormone produced by the stomach that reflects body weight changes and stimulates GH secretion. Recently, it has been shown to be associated with bone metabolism and eating behaviour. The underlying pathophysiology of adolescent idiopathic scoliosis (AIS) refers to possible abnormal bone development. AIS patients also frequently present with low BMI level.Eating behavioural disorders, endocrine disorders, abnormal grow...

Vascular calcification has a marked impact on arterial heterogeneity and plaque stability. Based on a cohort of 116 patients (carotid and femoral endarterectomies), the aim of the present study was to determine the influence of the bone-like vascular calcification and of osteoprotegerin (OPG) on plaque stability.Seventy-three carotid and 43 femoral plaques along with plasma were harvested and analyzed in a single center study. The presence of osteoid met...