Bone Abstracts

Introduction: For the very first time were assessed in vivo the metabolic and lipid profiles of osteocytes. During menopause period the appearance of an osteoporotic condition can be associated with an overall metabolic decline in bone cells, as well an increase of reactive oxygen species (ROS), and we hypothesized that it is mainly attributed to osteocytes metabolic and lipid changes, which could be apparently attenuated after raising blood estradiol (E2) levels. To ...

Introduction: Osteocytes play a major role in the bone remodelling unit (BRU). Thus, we hypothesize that mitochondrial bioenergetics impairment and mitochondrial/peroxisomal fatty acid β-oxidation unbalance is a cause of osteocytes metabolic decline during 17β-estradiol (E2) reduction. E2 and a phytochemical substitute, coumestrol (COU) were used (30 mg/kg during 24 h in ovariectomized rats in order to compare bone loss with sham-operated animal...

Background: Zoledronate, a third generation bisphosphonate, has showed high efficacy in the inhibition of bone resorption. The objective of this observational study was to assess short and long-term efficacy and safety of zoledronate in the treatment of active Paget’s disease (PD).Methods: Patients with active PD treated with zoledronate 5 mg were consecutively recruited. Clinical and laboratory parameters were determined before, at 3 and every 6 mo...

Biological calcification is a regulated process in which different types of tissues, cells and biomolecules participate in the coordination and regulation of the metabolic events involved in accumulating large amounts of calcium phosphate. This process could be speeded up using carbon nanotubes (CNTs) systems. The objective of our study was to compare cell growth and formation of mineralized matrix nodules by osteoblasts grown in plastic and in titanium (Ti) discs surfaces. Th...

The biomineralization process is initiated inside matrix vesicles (MVs), with phosphate and calcium ions crystallizing as hydroxyapatite. This process is accomplished by the activities of several proteins, such as annexins (e.g. AnxV) that mediates Ca2+ influx into MVs and tissue-nonspecific alkaline phosphatase (TNAP), a phosphomonohydrolase that uses ATP and PPi as substrates. Dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylserine (DPPS...

Despite the presence of skeletal anomalies in fetuses of female rats treated with caffeine, their effect on bone’s formation and growth have not yet been elucidated. The objective of this study was to evaluate the effects of caffeine on the formation and endochondral bone growth in rats. There had been used 36 Wistar rats distributed among the control group and others treated with caffeine at doses of 25, 50, and 100 mg/kg. Treated groups received caffeine daily throughou...

YB-1 (Y-box binding protein 1) is a multifunctional cold-shock protein that has been implicated in all hallmarks of cancer. Elevated YB-1 protein levels were correlated with poor prognosis in several types of cancers, including breast cancer (BC). In BC, high YB-1 expression is a marker of decreased overall survival (OS) and distant metastasis-free survival across all subtypes. YB-1 is also secreted by different cell types and may act as an extracellular mitogen. Therefore, ou...

In order to examine metabolic and biophysical parameters arising from obesity, male rats were given to drinking 30% sucrose (p/v) for 8 weeks. During the experimental period, animals in the control group (C) consumed higher amounts of food and water, but the body mass was smaller than the group receiving sucrose (S). In this group, the caloric load given to the animals for eight weeks resulted in increased energy consumption, in glycemia and in plasma leptin and abdominal fat....

Sclerostin, encoded by the SOST gene, functions as an inhibitor of the Wnt pathway and thus it is an important regulator of bone homeostasis. The fact that osteoblasts, the only cells expressing SOST, lay buried deeply in the bone matrix, poses intrinsic difficulties to the study of the regulation of this gene. Since RUNX2 and SP7/OSX are two known regulators of the differentiation of cells of the osteoblastic lineage, the aim of this study was to determine t...

Purpose: To evaluate whether calcium and vitamin D intake is associated with 25-hydroxyvitamin D (25(OH)D) and parathyroid hormone (PTH) serum concentrations or is associated with either the phalangeal dual energy X-ray absorptiometry (pDXA) or the quantitative bone ultrasound (QUS) at the phalanges and the calcaneus in independent elderly men from southwestern Spain.Methods: Serum PTH and 25(OH)D were measured in 199 healthy elderly men (mean age: 73.31...