Bone Abstracts

Bone tissue engineering relies on the combination of scaffolds and cells aiming to heal bone defects. Among the many candidates for scaffolds, gelatin sponge (Gelfoam®) and fibrin glue (Tissucol®) are of interest due to the well-established biocompatibility and the ability to be loaded with cells. Here, we evaluated their potential to stimulate bone formation by microtomography analysis. For this, 5-mm unilateral calvarial defect was created in rats deeply anesthetiz...

Introduction: Parathyroid hormone (PTH) measurement is of use in i) differential diagnosis of hypercalcaemia and ii) patients with renal impairment at risk of bone disease. PTH immunoassays are complicated by cross-reactivity with truncated (inactive) variant forms, which accumulate in patients with renal impairment. PTH assay variability is a critical governance issue in renal medicine, suggesting an MS-based reference method is required.Aim: To develop...

Introduction: The interaction between RANK/RANKL is critical for the regulation of osteoclastogenesis and bone resorption. Inhibition of this interaction helps restore the balance between bone resorption and formation. ALX-0141, a novel biological agent (Nanobody) that specifically targets RANKL, was studied in a Phase I trial to assess the safety, tolerability, immunogenicity and PK after single injection.Methods: Forty-two healthy postmenopausal women ...

Introduction: Vitamin D deficiency has been associated with impaired physical functioning and several chronic diseases and might thereby affect quality of life and self-rated health. The aim of this study was to assess relationships of serum 25-hydroxyvitamin D (25(OH)D) with quality of life and self-rated health, and to examine whether physical performance and number of chronic diseases mediate these relationships.Methods: Data were obtained from the LA...

Introduction: In postmenopausal women with vertebral compression fractures (VCF) the mechanisms regulating healing processes and an anti-osteoporotic treatment are not completely clarified. The aim of this prospective study was the evaluation of bone turnover markers, bone mineral density and radiographic progression of one or more VCF during assumption of risedronate, strontium ranelate or teriparatide.Materials and methods: Women with recent osteoporot...

Objectives: The interaction between RANK and its ligand RANKL is critical for the regulation of osteoclastogenesis and bone resorption. Inhibition of this interaction helps restore the balance between bone resorption and bone formation. ALX-0141, a novel biological agent (Nanobody) that specifically targets RANKL, was studied in a phase I trial to assess the safety, tolerability, immunogenicity and PK after a single s.c. injection.Methods: Forty-two heal...

Bisphosphonates are used to treat bone disease characterised by increased bone resorption by inhibiting the activity of mature osteoclasts, resulting in decreased bone turnover. Bisphosphonates may reduce the population of osteoclast precursor cells (OPCs). Our aims were to investigate the effect of bisphosphonates on i) OPCs and ii) bone turnover in postmenopausal women with osteoporosis compared with healthy premenopausal women. Participants were 62 postmenopausal women (mea...

The purpose of this study was to analyze the influence of diet supplemented with hydrosoluble soy extract – HSE in bone mass of male and healthy Wistar rats. Sixteen (16) 30 days old animals were kept in controlled conditions with light/dark cicle and fed with commercial food and filtered water. Randomized into two groups: control group received 500 ml of water and 300 g of food, daily; supplemented group S, in addition of water and food, they also received 500 ml of soy ...

MicroRNAs are important regulators of gene expression with documented role in bone metabolism and osteoporosis. Moreover, the use of miRNAs constitutes potential therapeutic targets. Our aim was to identify miRNAs differentially expressed in fractured compared to healthy bone. Additionally, we performed a miRNA profiling of primary osteoblasts to assess the origin of the differentially expressed miRNAs. Total RNA was extracted from fresh femoral neck trabecular bone from women...

Introduction: The FRAX tool can be used to assess absolute fracture risk, but the majority of treatment decisions are still based on measurements by DEXA. In remote rural Greek areas, after the socioeconomy crisis, access to the nearest DEXA facility became more difficult.Purpose: Role of combined QUS and FRAX in the identification of low risk patients who do not require DEXA applying a FRAX threshold of 4% for the 10-year major osteoporotic fracture pro...