Bone Abstracts

Objective: Minimally invasive parathyroidectomy (MIP) has become a frequently used strategy but it requires a precise preoperative localization and the use of intraoperative PTH to fulfill its benefits. The current localization techniques (ultrasonography, MIBI scan) have shortcomings and intraoperative PTH is not available in our country.Case presentation: We report the cases of two patients, females (63 and 55 years old), with clinical and biochemical ...

Bisphosphonates (BPs) are widely used as antiresorptive drugs. However, one of most potent BPs, zoledronic acid (ZA) can cause BP-related osteonecrosis of the jaws (BRONJ) with a poorly understood pathophysiology. The aim of this study was to find a clue for the development of BRONJ by evaluating the cytotoxic effects of ZA on osteoblasts and examining the action mechanism of ZA on osteoclast differentiation. Jaw bone osteoblasts (JB-OBs), long bone osteoblasts (LB-OBs), and b...

Background: The optimal level of 25-hydroxyvitamin D2 (25(OH) D) for a healthy bone is not clear, nor the effects of treatment with vitamin D. Few previous studies have measured treatment effect on BMD in young deficient individuals.Materials and methods: A treatment group of vitamin D deficient immigrant women and a control group of ethnic Swedish women were recruited during pregnancy. A treatment of 800–1600 IU cholecalciferol and 500–1000 mg...

Objective: Patan, is a semi urban area in Gujarat, India where fluorosis is endemic (Fluoride concentration in ground water 1.96–10.85 ppm, Patel et al., 2008). Exposure to fluoride is likely to be higher in lower socio-economic class (SEC) due to lack of access to bottled water. Calcium intake and vitamin D status may modify fluorine absorption. Therefore, the aims of this study were to examine: i) prevalence of dental and skeletal fluorosis in children from upper, middl...

Objectives: Previous studies have reported that the rise in parathyroid hormone (PTH) concentrations may be used to determine the levels of vitamin D adequacy. However, dietary calcium intake may also influence the rise in PTH. In the light of low calcium intakes in Indian adolescents, our aim was to investigate the relative importance of calcium intake in relationship of serum 25 hydroxyvitamin D (25OHD) with intact serum PTH in apparently healthy children.<p class="abste...

Background: Prader Willi syndrome (PWS) is a genetic disorder characterized by severe hypotonia in infancy, hypogonadism, dysmorphic features, early onset obesity and mild mental retardation.Although hip dysplasia occurs in 10–20% in patients with PWS, hip dislocation requiring surgery is seen rarely. To our knowledge, surgical treatment of hip dislocation in PWS has not been reported before.We present a case with PWS and hip ...

Several cell types form the hematopoietic stem cell niche. These niches sometimes become hijacked by cancer cells, which may later form metastatic lesions. Our aim was therefore to characterize the bone marrow microenvironment to affect cancer cell homing to bone marrow.Pharmacologic modification consisting of PTH to stimulate the osteoblasts and zoledronic acid to prevent the increase in osteoclasts through PTH resulted in increased homing of cancer cel...

Cancer cell growth is dependent on the microenvironmental support. Prostate and mammary cancer (PCa and MCa) cells preferentially metastasize to bone, where they induce either an osteoblastic or osteolytic response. These opposite stromal responses suggest that different types of cancers adopt distinct strategies to hijack the bone marrow/bone stroma for their growth support. However, the molecular cues underlying these divergent responses are largely elusive.<p class="abs...

Intrinsic quality, micro and nano structure of bone after 1 month of spaceflight and during 1 week of recovery had never been investigated. The aim was to explore these properties in appendicular and axial skeleton of mature mice.Ten C57/Bl6 male mice flew on the 30-day space Russian BION-M1 high-orbit satellite mission (Biomedical Ethics Commission of IBMP, n°319). Five were euthanized 12 h after landing, the others 8 days later. A ground control g...

We have previously shown that female neuronal nitric oxide synthase knockout (nNOS KO) mice have increased trabecular bone volume. However, this study was performed in mice at 10 weeks of age only. To investigate whether the high bone volume is maintained during ageing, we compared 3-month- and 12-month-old wild type (WT) and nNOS KO mice using μCT. The tibias from 8 WT and 8 nNOS KO mice at each age were dissected, fixed for 24 h in buffered formalin, stored in 70% ethan...