Bone Abstracts

Objectives: To characterize clinical features and long-term outcome of CRMO, a rare inflammatory bone disease of childhood and adolescence, which forms part of the spectrum of chronic non-bacterial osteomyelitis (CNO).Methods: We studied 33 patients with an established diagnosis of CRMO followed at the Centre for Bone Quality of the Leiden University Medical Center from 1994 to 2018. Demographic and clinical data were collected at presentation and last f...

Background: Primary tumoral calcinosis is an orphan disease. The data about the incidence of this disease, as well as clinical recommendations for treatment are not presented in the literature.Presenting problem: Two patients – 11.5 years old boy and 8 years old girl with primary tumoral calcinosis had multiple foci of the subcutaneous calcification, walking impossibility, wheel-chair condition, fatigue, high fever and equinus deformity of the left ...

RANKL induced osteoclastogenesis is mediated by several transcription factors such as NF-κB, AP-1 and Nfatc1. We have found that also cysteine proteinases are involved in the signaling pathway downstream RANK. Thus, cystatin C, Z-RLVG-CHN2 (the sequence of which is based upon one of the enzyme inhibitory domains in cystatin C) and the fungal molecule E-64 – inhibit RANKL induced mouse and human osteoclast formation in vitro (Strålberg et ...

Aims: To describe different multinucleated giant cell phenotypes and their associations with synovitis in people with rheumatoid arthritis (RA) or osteoarthritis (OA), and in an experimental model of chronic arthritis.Material and methods: A cross-sectional comparative study was performed on samples of knee synovia from age-matched patients with RA, OA, or non-arthritic post mortem (PM) controls. OA synovia were stratified by histological inflammation gr...

Peripheral quantitative computed tomography (pQCT) is receiving considerable attention in the diagnosis and monitoring of human bone diseases. It is well accepted that lower image resolution compared to micro-computed tomography (micro-CT) affects bone morphometry. With advances in micro-CT evaluation techniques such as sample-specific remodeling simulations or dynamic bone morphometry, there is the potential to also allow the application of such techniques to clinical pQCT sc...

Introduction: Optimal vitamin D intake for lactating women remains controversial. We hypothesized that 1200 IU/day (vs 400 IU/day) of vitamin D during breastfeeding will enhance maternal vitamin D status and bone mass.Methods: Healthy mothers after term, singleton delivery were randomized to receive vitamin D3: 1200 IU/day (800+400 IU/day from multivitamins) or 400 IU/day (placebo+400 IU/day from multivitamins) during lactation. Serum 25-hydroxyvitamin D...

Interleukin- 34 (IL34) is a new, challenging cytokine discovered in 2008. It promotes the proliferation, survival and differentiation of the monocyte/macrophage lineage with almost the same efficiency as the macrophage-colony stimulating factor (M-CSF). IL34 has already been described to play a key role in various musculoskeletal bone diseases such as bone giant cell tumors or in rheumatoid arthritis. These ‘twin’ cytokines share a lot of functional similarities whic...

Bone has recently emerged as a novel endocrine organ regulating glucose metabolism. Ectonucleotide pyrophosphatase/phosphodiesterase-1 (NPP1) controls bone mineralisation by generating the mineralisation inhibitor pyrophosphate. In clinical studies increased activity of NPP1 has been found in patients with insulin resistance, and it has been shown to directly inhibit the insulin receptor. We hypothesised that mice lacking NPP1 (Enpp1−/−) would exhibit im...

The switch from a cartilage template to bone during endochondralossification of the growth plate requires dynamic and close interaction between the cartilage and the developing vascular structures. Vascular invasion of hypertrophic cartilage, with blood vessels coming from the bone collar, serves to bring in osteoblast- andendothelial precursor cells along with chondroclasts and their precursors into future ossification centres of the growth plate.Potent...

Using a longitudinal observational study with two evaluations and a 1 year follow-up interval, we investigated the influence of everyday physical activity (PA) and skeletal geometry in bone mineral density (BMD) and bone mass distribution at the proximal femur (PF) in 96 girls and 81 boys (10–12 years). Whole body and left hip DXA scans were used to derive geometric measures of the pelvis (inter acetabular distance – IAD) and PF (abductor lever arm – ALA). BMD w...