Bone Abstracts

Titanium implants have been extensively used in dentistry as a replacement of absent dental elements. The biocompatibility of a material depends on cellular response in contact with a surface. The microarray technology is a tool to obtain an overview of the cell state in terms of large-scale transcriptional expression in different situations. Thus, the objective of this investigation was to evaluate the transcriptional profile of osteoblastic cells from human alveolar bone cul...

Aim: Aim of our study was to investigate the serum adiponectin, leptin, and osteoprotegerin (OPG) levels and its expressions in the adipose tissue, and their relationships with bone metabolism and in patients with severe C chronic obstructive pulmonary disease (COPD).Materials and methods: Serum leptin, adiponectin, OPG, the receptor activator of nuclear factor-kB ligand (RANKL), and bone turnover markers (osteocalcin and type 1 collagen C-telopeptide (C...

Mutations of the ALPL-gene are closely related to hypophosphatasia (HPP), an inherited disorder of bone and mineral metabolism with clinically heterogeneous symptoms. To date 278 different mutations have been described, leading to reduction or completely loss of enzymatic activity of the tissue nonspecific alkaline phosphatase (TNAP).We present the case of a 6-year-old boy with clinical features and laboratory results consistent with infantile H...

Introduction: Niemann–Pick disease (NPD) is a rare autosomal recessive lysosomal lipid storage disorder. It is caused by mutations of genes which products are involved in the metabolism of sphingolipids. Their dysfunction causes sphingomyelin to accumulate in different organs which leads to progressive multisystemic disorder. Types A and B NPD are caused by mutations in sphingomyelin phosphodiesterase-1 gene with deficiency of acid sphingomyelinase (ASM). Types C and D NP...

Vaspin (visceral adipose tissue-derived serine protease inhibitor) is a newly discovered adipokine that widely participates in diabetes mellitus, polycystic ovarian syndrome and other disorders of metabolism. However, the effect of vaspin on the regulation of osteogenesis and the mechanism responsible are still unclear. Here, we found that vaspin could attenuate the ALP activities, osteocalcin secretion and Runx2 expression in the preosteoblast cell line MC3T3-E1 in a dose-dep...

miRNAs are epigenetic regulators of gene expression, increasingly recognised as prominent regulators of bone metabolism. Following our in vitro studies of miRNAs profiles in osteoblasts we extended the research to human bone samples. Our aim was to find and functionally validate miRNAs differentially expressed in osteoporotic and osteoarthrotic bone with potential for targeting genes relevant in bone metabolism.Trabecular bone tissue samples wer...

Due to its huge socio-economic impact a better understating of osteoporotic fracture healing is crucial.Thirty-one female merino land sheep were randomly divided into four groups. (i) Untreated control-group (C, n=8); (ii) bilateral ovariectomy (OVX, n=7); (iii) OVX and calcium-deficient diet (OVXD, n=8); and (iv) OVXD and additional biweekly corticosteroid injections (OVXDS, n=8). Drill-hole defects (7.5 mm in diamete...

South African studies indicated that urban Black and White women have similar bone turnover. However, urban Black women had low bone mass and greater exposure to lifestyle risk factors for bone disease. The purpose of this prospective study was to assess changes in bone turnover, parathyroid hormone (PTH) levels, bone health (forearm, hip and lumbar spine bone mineral density) in urban black South African women over 3 years. Black, urban postmenopausal women (n=144, &...

Safety data on zoledronic acid (ZA) administration for osteoporosis suggest potential kidney toxicity; indeed, it is not indicated in patients with impaired renal function. Patients’ clinical monitoring usually includes glomerular filtration rate (GFR) evaluation; no study addressed the issue of the early kidney injury. We evaluated the early (within 3 months) effect of iv ZA on renal function defining the potential role of AKI biomarkers in unveiling subtle damage.<p...

MicroRNAs (miRNAs) are small non-coding RNAs that have emerged as critical post-transcriptional regulators of gene expression. There is increasing evidence that miRNAs play an important role in osteoblast commitment and differentiation. The main aim of this study was to identify and characterize novel microRNAs regulating osteoblast functions. We report the role of mmu-miR-1187 in osteoblast differentiation and the mode by which it regulates osteogenesis. MicroRNA profiling of...