Bone Abstracts

Background: Hypoxia induces mesenchymal stem cells (MSCs) proliferation but results in a population with impaired osteogenic differentiation potential. Bone healing of tibial lengthening is enhanced by hyperbaric oxygen therapy. However, little is known about the effects of HBO on the Wnt signaling pathway in MSCs.Materials and methods: MSCs were cultured in complete medium and the osteogenic groups were cultured in osteogenic induction medium. Control c...

Up to now, most of standard methods for measuring inorganic phosphate (Pi) to determine phosphatase activity are based on coupled enzyme assays, colorimetric methods or conductance measurements. However, none of these methods can measure Pi, substrate and protein concentrations simultaneously, allowing direct kinetic determinations of phosphatase activity in cells in a single assay. Among the enzymes having a pyrophosphatase activity and releasing Pi...

Background: The activation of Wnt/β-catenin signaling promotes cellular senescence and induces matrix metalloproteinases (MMPs) expression in intervertebral disc (IVD). However, little is known about the effects of hyperbaric oxygen (HBO) on the Wnt/β-catenin signaling and MMPs expression in degenerated human IVDs.Materials and methods: Nucleus pulposus cells (NPCs) were separated from the degenerated disc nucleus tissue by performing sequentia...

Background: Expression of toll-like receptor (TLR) 2 and 4 was up-regulated in lesional areas of OA cartilage. Hyperbaric oxygen (HBO) reduces the TLR signalling pathway in multiple organ failures. However, little is known about the effects of HBO on the catabolic pathway mediated by TLRs in human osteoarthritic (OA) chondrocytes.Materials and methods: OA chondrocytes were separated from the knees of OA patients by performing sequential enzymatic digesti...

Introduction: Physical exercises improve muscle strength, aerobic capacity and bone density in patients with rheumatoid arthritis (RA). However, some physicians fear to recommend the intensive exercises. The aim of the study is to compare the efficacy of two 6-month exercise programs in patients with early RA.Methods: Fifty-one patients with early RA were randomized into 3 groups. At hospital stage 15 patients underwent ten high-intensity dynamic exercis...

Background: Sarcopenic obesity is usually defined by the combination of sarcopenia and obesity but there is not a standard definition yet. The recommended method for the quantification of muscle mass is dual energy X-ray absorptiometry (DXA), based on assessment of total fat mass and appendicular lean mass. Low Body Mass Index (BMI) used to be considered as a risk factor for fragility fracture. However, recent evidences have demonstrated a correlation between BMI and vertebral...

Objectives: Hypophosphatasia (HPP) is a rare, inherited metabolic disorder caused by loss-of-function mutations in the ALPL gene that encodes the tissue-nonspecific alkaline phosphatase TNAP (ORPHA 436). Its clinical presentation is highly heterogeneous with a remarkably wide-ranging severity. HPP affects patients of all age. Therefor diagnosis is often difficult and delayed. To improve the understanding of HPP in children and in order to shorten the diagnostic time span in th...

Objective: The aim of this study was to assess the relationship between renal function (estimated glomerular filtration rate (eGFR) using Cockcroft–Gault (CG) equation and modification of diet in renal disease (MDRD), respectively), bone mineral density (BMD) and both arterial stiffness in Korean postmenopausal women.Materials and methods: From January 2008 until December 2010, among the person who got medical examinations including bone densitometr...

Extracellular nucleotides, signalling through P2 receptors, play a significant role in bone biology. ATP and ADP act via the P2Y1 or P2Y12 receptors to promote osteoclast formation and activity. Bone cells express the P2Y2 receptor and, in osteoblasts, it plays a role in regulating bone mineralisation. This investigation examined the role of the P2Y2 receptor in osteoclasts. Primary osteoclasts were isolated from the bone ma...

Background: Among body composition phenotypes there is no consensus on definition and consequences of sarcopenic obese phenotype. The recommended method to quantify the muscle mass is the dual energy X-ray absorptiometry (DXA), based on measurement of total fat mass and appendicular lean mass. A high BMI may provide a great bone mineral density (BMD) in many individuals, but it does not guarantee optimal bone strength. The aim of our study is to investigate the influence of sa...