Bone Abstracts

Introduction: Umbilical cord blood (UCB) is increasingly used in hematopoietic stem cell (HSC) transplantations; however, the low cell numbers are still remaining as a limiting factor for proper engraftment. Osteoblasts are major constituents of HSC niche and play important roles in regulating HSC self-renewal and differentiation. Recently, extracellular vesicles (EVs) have been implicated in stem cell fate regulation via horizontal transfer of proteins and nucleic acids betwe...

IGF1 is an osteo-anabolic factor that stimulates osteogenic precursor cell differentiation. IGF1 is produced in bone in response to mechanical stimulation, but also in mechanically-stimulated muscle cells. IGF1 enhances the rate of mRNA translation in muscle cells via activation of the PI3K/AKT/mTOR pathway, thereby increasing in muscle mass. Therefore we hypothesized that IGF1 not just enhances osteogenic differentiation of precursors, but also stimulates protein syn...

Introduction: Although systemic lupus erythematosus (SLE) has traditionally been considered a disease of women, men may also be afected. Male osteoporosis is increasingly recognised. Several studies have reported that mean bone mineral density is significantly reduced in SLE women patients and most of them have low levels of vitamin D. The aim of our study is to analize this situation in men.Objectives: Determine 25-hydroxyvitamin-D (25OHD) serum concent...

Many pharmacological treatments have been developed in order to prevent or treat postmenopausal osteoporosis. There is not any study in the literature comparing long-term treatments of osteoporosis. The aim of this work was to studythe effects of long-term prevention treatment with zoledronic acid (ZA) and strontium ranelate (SrR) on bone microstructure in ovariectomized rats.Sixty 6-month-old female Wistar rats were used in this study and divided into f...

During early life undernutrition impairs normal weight and height gain that affects bone health. During recovering it is required an extra-amount of Ca to ensure the high demands of bone mineralization. A mixture of galactooligosaccharides (GOS) and fructooligosaccharides (FOS) would be a useful tool to increase mineral intestinal absorption to enhancing the amount of Ca to be retained into bone. Our objective was to evaluate, in an experimental model of protein restriction (P...

see P462....

see P214....

In this study, we evaluated the effect of the association of a bioactive glass-ceramic scaffold (Biosca) with mesenchymal stem cells derived from either bone marrow (BM-MSCs) or adipose tissue (AT-MSCs) on bone formation in calvarial defects of osteoporotic rats. Wistar rats were submitted to bilateral ovariectomy (OVX) or only to the surgical stress (Sham), under approval of the Committee of Ethics in Animal Research. After 5 months, 5-mm unilateral calvarial defect was creat...

Background: Fibrodysplasia Ossificans Progressiva (FOP) is a progressive disease characterized by periods of heterotopic ossification, often in ligaments. The underlying mechanism is far from clear, partially due to limited access to patient-derived cell models. Periodontal ligament fibroblasts (PLF) from extracted teeth can potentially be used to study deviant bone remodelling processes in vitro since these cells are derived from actual ligaments. They further provid...

Hypoxia and the hypoxia-inducible factor 1alpha (Hif1alpha) are known to play critical physiological functions in endochondral bone development. However, their role in abnormal cartilage formation (chondrodysplasia) is unknown. Our goal was to test the possibility that altered oxygen homeostasis, which would result in abnormal Hif1alpha expression and activity, could lead to chondrodysplasia. This was done using matrix metalloproteinase (MMP) 9 and 13 deficient mice, which pre...