Searchable abstracts of presentations at key conferences on calcified tissues

ba0005p133 | Cancer and bone: basic, translational and clinical | ECTS2016

Novel evidence that ApoA-1 deficiency facilitates HSC mobilization and differentiation and halts HSC quiescence and self-renewal in mice

Mastora Ioulia , Papachristou Nicholaos , Blair Harry , Kalyvioti Elena , Syggelos Spyros , Karavia Eleni , Kypreos Kyriakos , Papachristou Dionysios

Recent evidence suggests that osteoblastic bone marrow niche is vital for the maintenance and self-renewal of hematopoietic stem cells (HSC). It has been recently proposed that cholesterol efflux pathways participate in HSC mobilization and that cholesterol-sensing pathways control the proliferation of HSC progenitors. Moreover, we have recently documented that HDL perturbations result in impaired osteoblastic function in mice. In the present study, we aimed at investigating t...

ba0005p260 | Muscle, physical activity and bone | ECTS2016

Effects of calcifediol versus cholecalciferol on 25(OH)D3 serum levels, appendicular muscle strength, and physical performance in post-menopausal women

Moretti Antimo , de Sire Alessandro , Calafiore Dario , Gimigliano Raffaele , Gimigliano Francesca , Iolascon Giovanni

Background: Post-menopausal women generally present reduced serum levels of vitamin D, reduced VDR expression in skeletal muscle cells, and a gradual loss of muscle mass and muscle function. The relationship between serum 25-hydroxyvitamin D [25(OH)D3] levels and muscle strength has been extensively investigated, even though there is no agreement in literature. Therefore, aim of our study was to evaluate the effects of vitamin D on 25(OH)D3 levels, muscle strength, and physica...

ba0005p317 | Osteoporosis: evaluation and imaging | ECTS2016

Cellular and extracellular investigations of healing parameters in a sheep model of osteoporosis

Schaefer Annemarie , Rosch Sebastian , Weisweiler David , Boecker Wolfgang , Lips Katrin S , Heiss Christian , Malhan Deeksha , Khassawna Thaqif El

Due to its huge socio-economic impact a better understating of osteoporotic fracture healing is crucial.Thirty-one female merino land sheep were randomly divided into four groups. (i) Untreated control-group (C, n=8); (ii) bilateral ovariectomy (OVX, n=7); (iii) OVX and calcium-deficient diet (OVXD, n=8); and (iv) OVXD and additional biweekly corticosteroid injections (OVXDS, n=8). Drill-hole defects (7.5 mm in diamete...

ba0005p321 | Osteoporosis: evaluation and imaging | ECTS2016

The T-score standard in osteoporotic sheep model: insight into independent reference group

Khassawna Thaqif El , Engelhardt Markus , Weisweiler David , Bocker Wolfgang , Schmitt Jakob , Heiss Christian

FDA guidelines recommend the sheep as large animal model for osteoporosis. Studies of osteoporosis induction use dual energy x-ray absorptiometry (DEXA) to evaluate bone status. Experimentally, the T-score is calculated based on reference bone mineral density (BMD) values of animals before treatment (0 M). Whereas clinically the reference BMD represents values of an independent group of patients around 30 years old. The study hypothesize that the use of additional ind...

ba0005p463 | Other diseases of bone and mineral metabolism | ECTS2016

Change in bone mineral density with high-dose prednisone in patients with rheumatoid arthritis

Rasch Linda , van Tuyl Lilian , Kremer Martijn , Bultink Irene , Boers Maarten , Lems Willem

Background: Recently, we showed that treatment with COBRA-light therapy including prednisone with initially 30 mg/day was as effective as the original COBRA scheme, with initially 60 mg/day (Ter Wee, ARD 2015), in the treatment of rheumatoid arthritis (RA). Since high-dose glucocorticoids are associated with bone loss, we investigated the differences in bone mineral density (BMD) after 1 year of treatment in both arms.Objective: To determine whether ther...

ba0006oc2 | (1) | ICCBH2017

Methylation patterns at the novel DMR of GNAS (GNAS-AS2) in pseudohypoparathyroidism 1B (PHP1B or iPPSD3) subtypes

Hanna Patrick , Rochtus Anne , Jueppner Harald , Mackay Deborah , Francou Bruno , Bouligand Jerome , Mantel Anne , Anagnostou Elli , Vlachopapadopoulou Elpis , Gaillard Dominique , Delemer Brigitte , Linglart Agnes

PHP1B -iPPSD3 per the new proposed classification- is a rare disorder characterized in most patients by proximal tubular resistance to PTH resulting in hypocalcemia, hyperphosphatemia and elevated PTH. Loss-of-methylation (LOM) at the Differentially Methylated Region (DMR) at GNAS exon A/B occurs in all PHP1B patients, but methylation changes at other DMRs within GNAS occur in some familial and most sporadic PHP1B cases. All patients with autosomal dominant PHP1B (AD-...

ba0002p103 | (1) | ICCBH2013

Celiac disease and bone disorders: diagnosis age influence

Brunetto Oscar , Cau Ana Arias , Insua Claudia , Marzet Chriatian Boggio

Celiac disease (CD) is a frequent cause of malabsortion in childhood and affects calcium and vitamin D absorption.Aim: Analyze the vitamin D levels and bone mineral density (BMD) at diagnosis in patients affected with CD, and the influence of the age of diagnosis in the clinical presentation.Patient: 52 patients (female: 26) were evaluated.Results: The percentage with vitamin D deficiency (<20 ng/ml) was ...

ba0002p157 | (1) | ICCBH2013

Growth and bone health after hematopoietic stem cell transplantation or tyrosine kinase inhibitors in children with chronic myeloid leukemia: a single institution experience

Dilley Kimberley , Broglie Larisa , Chaudhury Sonali , Hijiya Nobuko

Objective: To examine the impact on growth and bone mineral density (BMD) of tyrosine kinase inhibitors (TKI) vs hematopoeitic cell transplant (HCT) for treatment of chronic myelogenous leukemia (CML) in patients <18 years of age.Methods: We performed a retrospective review of children with CML in chronic phase treated between 1992 and 2011 at a single institution and evaluated available growth and BMD data.Results: Twenty-five...

ba0002p163 | (1) | ICCBH2013

Phenotype–genotype correlation and role of ancillary investigations in atypical and rare forms of osteogenesis imperfecta

Balasubramanian Meena , Parker Michael , Bishop Nicholas J

Background: Osteogenesis imperfecta (OI) is a heterogeneous group of inherited disorders of bone formation, resulting in low bone mass and an increased propensity to fracture. It is a variable condition with a range of clinical severity. About 90% of patients with a clinical diagnosis of OI have a mutation in the COL1A1 or COL1A2 genes, which shows an autosomal dominant pattern (AD) of inheritance. Other genes are associated with the autosomal recessive (AR) ...

ba0004p32 | (1) | ICCBH2015

Characteristics of Malawian children undergoing corrective bone surgeries of rickets-like lower limb deformities

Braithwaite Vickie S , Greenwood Carla L , Bishop Nicholas J , Cashman John , Prentice Ann

Cases of non-vitamin D deficiency rickets have been reported in African countries including The Gambia, South Africa and Nigeria where the likely aetiology is a chronically low dietary calcium intake. Additional aetiological factors in Gambian studies are iron deficiency leading to a disruption in phosphate metabolism.Surgical correction of pathological rickets-like lower-limb deformities is the most common operation performed at the Beit Cure Orthopaedi...