Bone Abstracts

Objectives: Bone remodeling and maintenance require a fine balance between bone formation of osteoblasts and resorption of osteoclasts. Therefore, various skeletal disorders cause by imbalanced differentiation and activities of these cells. Receptor activator of NF-κB ligand (RANKL) induces Ca2+ oscillations and activates nuclear factor of activated T cells 1 (NFATc1) during osteoclast differentiation. Although Ca2+ oscillations play a key role for o...

This study examined the anti-osteoclastogenic effect of n-butanol extracts of Panax notoginseng on the receptor activator of NF-kB ligand (RANKL) induced RAW264.7 cells. Panax notoginseng is commonly used to treat chronic liver disease. Notoginseng has many beneficial effects, such as the suppression of liver fibrosis and anti-cancer activities. Notoginseng contains several biologically active components, such as ginsenosides Rb1, Rg1...

Objective: Bone metabolism was studied in patients with phosphate diabetes before and after medication.Methods: 39 patients with phosphate diabetes age 2–18 years (7.8±4 years, 64% male) and multiplanar deformities of the lower extremities were treated with monthly vitamin D (2000 IU/day), alfacalcidol (0.25 mg/every other day) and calcium glycerophosphate (600 mg/day) for 6 months.Biochemical indices were evaluated befor...

Histomorphometry is often adopted as a methodological approach in research of femoral bone structure. However, it is common do not describe technical details of procedure especially on the steps of macroscopy, paraffin embedding and microtomy. So we propose a simplified, reliable and reproducible method of histological processing for intertrochanteric region and femoral neck of mice, to ensure the achievement of tissue sections with similar structures, cell populations and his...

Arterial calcification is now considered as an actively regulated process resembling osteogenesis orchestrated by a number of systemic or constitutively expressed mediators. Genetic studies of rare inherited syndromes have identified key regulators of arterial calcification. Based on the pathogenic principles causing the diseases these can be classified into three groups:i) Disorders of an increased extracellular inorganic phosphate/inorganic pyrophospha...

Objectives: i) Describe bone mineral density (BMD) of children with MPS IV (Morquio disease), a rare genetic disorder which produces skeletal deformity, small stature and results in physical limitations such as the ability to walk.ii) Examine fracture history and factors affecting bone health in Morquio.iii) Describe technical issues encountered in assessing BMD by DXA in Morquio.Methods: In this prospective ...

Spinal cord injury (SCI) has been associated with marked bone loss and an increased risk of fractures under the SCI level. The aim of the study was to analyze the effect of recent complete SCI on bone turnover and bone mineral density (BMD) evolution and factors related to bone loss.Methods: Prospective study including patients with complete motor SCI (ASIA A or B) (<6 months). Bone turnover markers (bone formation: P1NP, bone ALP and resorption: sCT...

Osteogenesis imperfecta (OI) classification has recently been broadened to include genes that primarily affect osteoblast differentiation. TMEM38B encodes TRIC-B, a ubiquitously expressed monovalent cation-specific channel protein involved in calcium release from the endoplasmic reticulum. How alterations in TMEM38B cause OI remains poorly understood and bone matrix characteristics in affected patients have not previously been described.<p class="abstext"...

Objectives: As the receptor activator of the nuclear factor κB (RANK)/receptor activator of the nuclear factor κB ligand (RANKL)/osteoprotegerin (OPG) cytokine system is essential for osteoclastogenesis and Wnt signaling pathway for osteoblastogenesis, we decided to assess these two main bone regulatory pathways in patients with malignant bone tumors on the completion of anticancer therapy.Methods: The study included 35 patients (median age 15....

Objectives: To assess the effects of short-term vitamin D supplementation on bone metabolism, glycaemic status and immune function in vitamin D deficient children.Method: Treatment with daily 5000 IU cholecalciferol supplementation for 6 weeks. At baseline and end of treatment serum 25 hydroxyvitamin D (25(OH)D), parathyroid hormone (PTH), alkaline phosphatase (ALP), serum collagen type 1 cross-linked C-telopeptide (CTX), serum calcium, HbA1c, sex hormon...