Searchable abstracts of presentations at key conferences on calcified tissues

ba0003pp38 | Bone development/growth and fracture repair | ECTS2014

Development and characterization of novel biodegradable scaffold materials

Fowler Tristan , Kampleitner Carina , Poocza Leander , Markus Andrea , Dullin Christian , Hildebrand Gerhard , Alves Frauke , Liefeith Klaus , Hoffmann Oskar

The development of optimally performing biomaterials mimicking the natural physiological processes underlying bone repair is essential for non-healing large bone lesions. We sought to develop scaffolds that fill the lesions and improve bone healing. We tested three scaffolds comprised of varying percentages of lactide (LA), caprolactone (CL), and methacrylate produced by two-photon photopolymerization; LCM 3 (8:2; 85%), 4 (9:1; 90%), and 6 (9:1; 40%). We hypothesized that the ...

ba0003pp101 | Cancer and bone: basic, translational and clinical | ECTS2014

The effect of granulocyte colony-stimulating factor in adults undergoing autologous peripheral blood stem cell collection

Amrein Karin , Drexler Camilla , Oczko Susanna , Sareban Nazanin , Sipurzynski Sabine , Pieber Thomas R. , Lanzer Gerhard , Wagner Doris , Fahrleitner-Pammer Astrid

Introduction: Granulocyte colony-stimulating factor (G-CSF) is widely used to mobilize peripheral blood stem cells (PBSC) and enable PBSC collection by apheresis. Although bone pain is a common adverse event following G-CSF treatment, little is known on its effect on bone metabolism.Methods: Markers of bone turnover (OC, osteocalcin, β-CTx, bALP, C-terminal telopeptide of type I collagen, bone specific alkaline phosphatase, TRAP, tartrate resistant ...

ba0005p73 | Bone development/growth and fracture repair | ECTS2016

Combination of novel two-photon photopolymerised scaffolds and bioactive elastin-like-recombinamers induce bone regeneration

Kampleitner Carina , Hildebrand Gerhard , Liefeith Klaus , Gonzalez Constancio , Rodriguez-Cabello Jose Carlos , Markus Andrea , Dullin Christian , Alves Frauke , Hoffmann Oskar

Non-healing fractures caused by trauma, disease or tumour resection demand the use of bone grafts to support and stimulate the healing process. We sought to develop and evaluate the effect of a novel bioactive biodegradable biomaterial designed to fill large bone lesions and to improve bone healing. We used two-photon polymerised synthetic polymer scaffolds composed of lactide (LA), caprolactone (CL), and methacrylate (MA) with varying LA: CL ratio and percentage of methacryla...

ba0002is10 | The fracturing child: therapeutics | ICCBH2013

Medical therapies: present and future

Munns Craig

Bisphosphonates are the mainstay of medical therapy in the fracturing child with osteoporosis. The majority of the data in children pertains to i.v. pamidronate use in children and adolescents with osteogenesis imperfecta (OI), where pamidronate has been associated with improvements in bone mineral density, cortical thickness, vertebral shape, pain, mobility and height.1 Side-effects of pamidronate including acute phase response to the initial dose and retardation o...

ba0002p154 | (1) | ICCBH2013

Smad4 regulates growth plate chondrocyte proliferation, columnar organization and proteoglycan synthesis

Whitaker Amanda , Berthet Ellora , Cantu Andrea , Laird Diana , Alliston Tamara

Objective: The physis, or growth plate, is comprised of precisely organized chondrocytes that confer longitudinal growth of the bone. Multiple signaling pathways cooperate to regulate growth through their control of chondrocyte shape, polarity, proliferation, and differentiation.1,2 Disruption of these cellular events result in physeal defects, skeletal deformities, and abnormal limb growth. Loss of function mutations in Smad4, a common intracellular effector of all...

ba0004p106 | (1) | ICCBH2015

Metatropic dysplasia is associated with increased fracture risk and increased markers of bone turnover

Bober Michael , Duker Angela , Carney Megan , Ditro Colleen , Rogers Kenneth , Mackenzie William

Metatropic dysplasia (MD) was first described by Maroteaux et al. in 1966. Its name was derived from the Greek word metatropos which means ‘changing pattern’ because individuals with this diagnosis begin life with a short-limbed dysplasia and a long trunk with narrow chest, and over time their dysplasia becomes short-trunked due to progressive kyphoscoliosis1. It is now recognized that MD is caused by gain-of-function mutations in transie...

ba0005p18 | Biochemical testing | ECTS2016

Atorvastatin effects on a glucocorticoid-induced osteoporosis animal model

Pacheco-Pantoja Elda , Rojano-Carrillo Brahim , Mateo-Moguel Pablo , Dominguez-Angulo Cindy , Aguilar-Aleman Gustavo , Lopez-Rivas Victor

Osteoporosis is a chronic disease with a high prevalence in older adults. There is evidence that statins have some beneficial effects on bone metabolism. Although the trial reports are ambiguous, it is now accepted that statins block the osteoclast differentiation inhibiting to some extent bone resorption. Our aim was to evaluate the effects of a statin in an animal model, which was induced to osteoporosis by glucocorticoids and then treated with atorvastatin. The experimental...

ba0005p74 | Bone development/growth and fracture repair | ECTS2016

In vitro investigation of the effect of Magnetic Resonance guided Focused Ultrasound Surgery on osteosarcoma cell lines

Agnese Valentina , Costa Viviana , Carina Valeria , Bellavia Daniele , Raimondi Lavinia , De Luca Angela , Scoarughi Gianluca , Midiri Massimo , Stassi Giorgio , Giavaresi Gianluca

Aim: Magnetic Resonance guided Focused Ultrasound Surgery (MRgFUS) is an invasive treatment able to control local disease and pain of bone tumors. Unfortunately, there is not any scientific evidence of the biological effect of MRgFUS treatment on tumor cells, especially in lower dose region, where tissues are only warmed to sub-lethal temperatures. Here we investigate the effect of in vitro MRgFUS treatment, at different levels of acoustic energy (200–630 J), on ...

ba0001oc1.1 | Osteoporosis epidemiology and long term treatment complications | ECTS2013

Disease-specific perception of fracture risk and incident fracture rates among postmenopausal women: findings from the Global Longitudinal Study of Osteoporosis in Women (GLOW)

Gregson Celia , Dennison Elaine , Compston Juliet , Adami Silvano , Adachi Jonathan , Anderson Frederick , Boonen Steven , Chapurlat Roland , Diez-Perez Adolfo , Greenspan Susan , Hooven Frederick , Lacroix Andrea , Nieves Jeri , Netelenbos J Coen , Pfeilschifter Johannes , Rossini Maurizio , Roux Christian , Saag Kenneth , Silverman Stuart , Siris Ethel , Watts Nelson , Wyman Allison , Cooper Cyrus

Patients with improved health understanding have greater autonomy over, and motivation towards, health-related lifestyles. We compared self-perceived fracture risk and 3-year incident fracture rates in postmenopausal women for a range of co-morbid diseases using data from the Global Longitudinal study of Osteoporosis in Women (GLOW).GLOW is an international cohort study involving 723 physician practices across 10 countries in Europe, North America, Austr...

ba0001pp283 | Genetics | ECTS2013

Discovery and replication of several loci significantly associated with lean body mass: a large meta-analysis of genome wide association studies (GWAS) from the ‘charge’ and ‘gefos’ consortia

Kiel Douglas P , Yerges-Armstrong Laura M , Hsu Yi-Hsiang , Stolk Lisette , Karasik David , Loos Ruth J F , Gudnason Vilmundar , Smith Albert , O'Connell Jeffrey R , Fu Amish , Fu Mao , Streeten Elizabeth A , Cauley Jane A , Robbins John A , Psaty Bruce , Johnson Toby , Kutalik Zoltan , Mitchell Braxton D , Livshits Gregory , Harris Tamara B , Ohlsson Claes , Zillikens M Carola

Introduction: The creatine kinase (CK) is a dimeric enzyme, involved in energetical metabolism. It is present in many tissues, but higher concentration in skeletal and cardiac muscle.Therefore, conditions that involve muscle tissue may increase this serum enzyme. Such enzyme elevation is usually observed in inflammatory myopathies and others autoimmune diseases.Sometimes some elevation in CK is not fully understood out off these co...