Bone Abstracts

RANKL induced osteoclastogenesis is mediated by several transcription factors such as NF-κB, AP-1 and Nfatc1. We have found that also cysteine proteinases are involved in the signaling pathway downstream RANK. Thus, cystatin C, Z-RLVG-CHN2 (the sequence of which is based upon one of the enzyme inhibitory domains in cystatin C) and the fungal molecule E-64 – inhibit RANKL induced mouse and human osteoclast formation in vitro (Strålberg et ...

Background: Recently, the prevalence of osteoporosis and sarcopenia in the elderly has dramatically increased. However the relationship between theses disease is not clear.Object: We aimed to determine the independent relations of muscle mass to osteoporosis (femur neck) in relation to body weight, fat mass, and other confounders.Design: We analyzed body composition and BMD data of 570 males and 734 females who are older than 65 ye...

Purpose: Denosumab is a new potent antiresorptive treatment of osteoporosis that can potentially induce a compensatory increase of parathyroid hormone (PTH) levels. We aimed to evaluate the alteration of PTH 1 and 6 months after denosumab’s administration (60 mg) with different regimens of calcium and vitamin D (Ca/D) supplementation, as well as the association of PTH with serum Ca and bone markers.Methods: This was a prospective, multicenter, study...

Many reports have proposed significant role of vitamin D on immune mediated disease. This study analyzed lymphocyte subsets according to serum 25(OH) vitamin D levels in postmenopausal women to determine the potential effect of vitamin D on immune-mediated disease.On a prospective observational basis, we enrolled 31 postmenopausal women who underwent health checkup in our hospital. Peripheral blood drawn for the estimation of total T, B cell, NK cell cou...

Eldecalcitol (ED-71; ELD), a 2β-hydroxypropyloxy derivative of 1α,25(OH)2D3, was approved to treat osteoporosis in Japan in 2011. The endothelial protective effect of vitamin D3 in osteoporosis is not clear. This study evaluated the endothelial protective effect of ELD in ovariectomized (OVX) rats.ELD (20 ng/kg) was orally administered five times a week for 4 weeks from 1 day after OVX surgery. Four weeks after surgery, flow-mediated dilation (...

Renal osteodystrophy encompasses the bone histologic abnormalities seen in patients with chronic kidney disease (CKD). The bone-specific alkaline phosphatase (BALP) isoform B1x is exclusively found in serum of some CKD patients. The aim of this study was to examine the relationship between serum BALP isoforms and histologic abnormalities of bone in patients with CKD on chronic dialysis (CKD-5D).Anterior iliac crest bone samples from 40 CKD-5D patients we...

Context: As bone metabolic markers, recent studies indicated vitamin D and osteocalcin (OC) were also involved in energy metabolism. While the exact relationship between vitamin D and OC in regulating energy metabolism is unclear. One of the possibilities is that: low vitamin D could lead to secondary hyperparathyroidism and following high bone turnover, which would enhance the quantity of bioactive OC.Objective: Our aim was to study whether OC mediated ...

Optineurin (OPTN) is a protein encoded by the OPTN gene. This protein is involved in several cellular mechanisms such as autophagy, NF-κB signaling, cellular morphogenesis, membrane and vesicle trafficking, and transcription activation. Mutations in OPTN have been described in glaucoma, amyotrophic lateral sclerosis and other neurological diseases. More recently, a polymorphism in this gene was also identified by a genome wide association study to be ass...

Bone fractures may depend on Vitamin D Receptor Gene (VDR), bone mineral density, bone turnover markers.Patients and methods: About 161 patients were recruited and underwent: skeletal densitometry (DXA) method and bone turnover studies (Osteocalcin and Ntx).The study group was evaluated using restriction enzyme digestion at BsmI (rs1544410), FokI (rs2228570), ApaI (rs7975232) and TaqI (rs731236), polymorphic sites of t...

This study is focused on the effects of the synergic use of vitamins D and K on human osteoblasts primary cultures derived from osteoporotic and normal patients. The aim of this work is the evaluation of the different cellular behaviour in response to the lipophilic vitamins stimulation. We included 20 osteoporotic and 20 control patients in age between 35 and 50 and in age between 55 and 85. All patients gave informed consent for using bone samples as a source of bone cells. ...