Bone Abstracts

Diabetes leads to development of osteoporosis. Experimental type 1 diabetes may be induced by a single dose of streptozotocin (STZ), and nicotinamide (NA) administered 15 min before STZ dose-dependently protects against the STZ action. Coffee drinking, apart of its health benefits, is taken into consideration as an osteoporosis risk factor. Data from human and experimental studies on coffee and caffeine effects on the skeletal system are inconsistent. For example, although oth...

Contemporary elemental imaging techniques are greatly contributing into the bone research. Synchrotron radiation induced confocal micro x-ray fluorescence technique (SR-μXRF) was employed for the analysis, being the most powerful and sufficient tool in detection and characterization of trace element distributions in bone tissue. Manganese (Mn), as a potential contributor into the mechanisms of calcium incorporation into bone tissue is in the spotlight of research. Further...

Since their initial discovery, microRNAs (miRNAs) have emerged as critical post-transcriptional regulators of gene expression that are able to modulate bone remodeling. Nonetheless, despite the peculiar and aggressive phenotype of pagetic osteoclasts and the associated increase in osteoblast activity, whether deregulation of miRNAs is involved in Paget’s disease of bone (PDB) remains unknown. Here, we performed a serum miRNA expression profile (Taqman Human MicroRNA Array...

Objectives: Chronic non-bacterial osteomyelitis (CNO) is a heterogenic group of immune-mediated inflammatory bone diseases with unclear pathogenesis. Only a few genes associated with this condition have identified. The aim of the study was to evaluate the spectrum of mutations in genes associated with primary immunodeficiency syndromes (PIDs) and autoinflammatory diseases (AIDs) in the cohort of patients with early-onset CNO from North Caucasus (Dagestan and Chechnya) and asse...

Background/aim: X-linked hypophosphatemia (XLH) is a rare disease characterized by low phosphate level. Scientific evidence points to link between hypophosphatemia and obesity in general population. The aim of our longitudinal observational study was to investigate the prevalence of obesity and associated factors in a large cohort of children with XLH.Patients/methods: We selected 172 XLH-children of 5–20 years (113 girls/59 boys). Anthropometric pa...

The identification of markers indicative of pathological FMS plays a pivotal role in the prevention of ACH complications.Objective: Identify key cranio-cervical junction neuroradiological features for the surgical choice and for the decompression outcome.Methods: Out of 191 ACH patients, we selected 24 patients before 4 years of age, who performed a first brain MRI and/or CT. Patients were divided into 2 groups: surgically treated ...

Background and aims: Bone turnover markers (BTMs) are used in clinical practice for assessing patients with osteoporosis and their treatment. In Spain it is necessary to fine-tune the reference intervals, since they were established years ago in a low number of individuals. The aims of this study were to establish robust reference intervals for BTMs in healthy young premenopausal Spanish women and to investigate the factors influencing BTMs.Methods: 185 ...

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Objective: Wnt/β-catenin pathway is a main regulator of bone remodeling, but might be inhibited in cartilage in osteoarthritis (OA). We here investigated the effect of mechanical loading in Wnt activation and the expression of Wnt antagonists in the joint tissues.Methods: Topgal mice were used. Mice underwent partial meniscectomy (Mnx) and sacrificed at 4, 6, and 9 weeks. Dissected knees were scanned by microCT and then prepared for cryosectioning t...

Aim: Wnt/β-catenin pathway promotes cartilage breakdown in osteoarthritis. We have previously shown that sclerostin preserves chondrocyte maintenance in vitro by reducing chondrocyte catabolism through the inhibition of Wnt/β-catenin pathway. However, sclerostin restores partially the expression of the anabolic genes. We therefore investigated the effect of sclerostin in the activation of Wnt non canonical pathways mediated by Ca2+/CaMKII, JNK, an...