Bone Abstracts

Introduction: One third of patients in the long-term heparin therapy show reduction in bone density. We have shown that heparin binds to bone morphogenetic protein 6 (BMP6) and inhibits its osteogenic activity in vitro. Here we explored whether heparin effects BMP6 mediated bone efficacy in vivo.Methods: We have used a mouse model of postmenopausal osteoporosis and tested the effect of heparin on BMP6 therapy and its osteogenic activity...

Apart from analyses of well-known correlates of age-related hip fracture risk, such as low BMD, impaired external geometry and deteriorated micro-architecture, there is increasing interest to elucidate nano-structural determinants of fracture risk at the bone mineralized matrix level.In this study we analyzed cortical bone specimens of the femoral neck region in five elderly women who sustained hip fracture and in four healthy women of corresponding age....

Tooth fracture is considered as a major problem in dentistry. As it is commonly observed in dental practice, one of the main factors that lead to increased tooth fragility is its devitalization. However, there is no definite mechanistic explanation for such phenomenon. We hypothesize that the possible response to this matter lies in the changes that occur in dentin due to altered microenvironment after endodontic procedure. Therefore, in this study we analyzed the structural a...

Hepatic osteodystrophy is an important complication of chronic liver disease associated with fractures resulting in pain, deformity and immobility. The aim of the study was to examine association of severity of alcoholic liver cirrhosis with areal bone mineral density (BMD) and to estimate bone geometric strength of the proximal femur in those patinets. The study included 27 male patients with alcoholic liver cirrhosis and control group of 36 healthy patients. Laboratory testi...

Objective: To establish the difference between deficiency, insufficiency and normal results of vitamin D according to severity of motor function disorder and the level of bone mineral density in children with cerebral palsy.Methods: Investigation encompassed 23 children, both genders, between 6 and 17 years of age, with diagnosed cerebral palsy, who were hospitalized between January 1 and December 31, 2014. Bone mineral density (BMD) was established usin...

Objective: The aim of the study was to establish effect of nutritional status on bone mineral density in children with cerebral palsy.Methods: The nutritional status was established in 23 children, both genders, with cerebral palsy, who were hospitalized at the Clinic for children habilitation and rehabilitation and at the, ‘Veternik’ Home, between January 1 and December 31, 2014. Evaluation of nutritional status has been done based on BMI. BMI...

Objective: Investigate bone mineral density in children with cerebral palsy (CP) with special focus on severe forms of CP.Methods: Investigation encompassed 23 children, both genders, between 6 and 17 years of age, with diagnosed cerebral palsy, who were hospitalized between January 1 and December 31, 2014. Bone mineral density (BMD) was established using Dual-energy x-ray Absorptiometry – DXA method, at L1–L4 lumbar vertebrae and at the femur ...

Although bone morphogenetic protein 6 (BMP6) is known for its ability to induce growth of bone and cartilage, recent studies identified BMP6 as a key endogenous regulator of hepcidin expression and iron metabolism. Here, we examined BMP6 presence in the serum and investigated whether circulating levels of BMP6 may reflect body iron status. First, we showed by liquid chromatography–mass spectrometry (LC–MS) and western blotting that BMP6 is present in the circulation ...

Elevated levels of circulating serotonin have been reported to decrease bone mineral density1. Conversely, reduced serotonin (5HT) in mice lacking TPH1, the rate limiting enzyme for 5HT synthesis, was reported to be anabolic to the skeleton with high osteoblastic activity2. However, in other studies TPH1 deletion led to either an initial increase in BMD due to inhibition of osteoclastic bone resorption3, or had no bone effect4</su...

BMP6 is a member of the TGFβ superfamily with a high potential to induce new bone formation. Recently, we discovered that blood coagulum from the patient’s own blood (WBCD) modified with calcium salts serves as an appropriate autologous carrier for BMPs (OSTEOGROW device). Preclinical experiments indicate that BMP6 is efficacious when used in a significantly lower amounts than BMP2 and BMP7. Besides testing its osteogenic activity, we also tested the influence of the...