Bone Abstracts

Objective: Children with hypophosphatasia (HPP) treated with asfotase alfa in a Phase 2 study (NCT00952484) and its open-label extension (NCT01203826) experienced significant improvements in skeletal mineralization and physical function that were sustained through 5 years of treatment (1). Herein, we report data from these studies with a maximum of 7 years of treatment.Methods: Children with HPP aged 6–12 years at baseline received asfotase alfa (3 ...

Objective: To evaluate safety and efficacy after 5 years of treatment with asfotase alfa in adolescents and adults with hypophosphatasia (HPP) in a Phase 2, open-label, randomized, dose-ranging study (NCT01163149).Methods: Treatment with subcutaneous asfotase alfa 0.3 or 0.5 mg/kg per d was compared with no treatment (control) for 6 months in patients aged 13–66 years. After 6 months, all patients (treatment and control groups) received active treat...

see PP448....

Relatively little is known about immediate consequences of continuing vs interrupting long-term bisphosphonate treatment. This report describes changes in bone turnover and BMD in a 1-year, dose-finding trial of the calcium-sensing receptor antagonist MK-5442 in postmenopausal, BP-treated women, randomized to continued alendronate 70 mg weekly, switch to placebo, or switch to MK-5442. Recruited women (n=526) had taken alendronate for ≥12 months and an oral BP fo...

Background and Objectives: Rheumatoid arthritis (RA) is a chronic disease characterized by severe bone destruction which has been associated with altered lipid metabolism. Apolipoprotein E (Apo E) is a lipoprotein mainly produced by macrophages. ApoE has been described as crucial in lipid metabolism but also as an important anti-inflammatory mediator regulating innate immunity and bone turnover. In the present study we investigated the role of Apo E in bone destruction during ...

Introduction: Recent data suggest that imbalances in lipid metabolism affect bone cell function resulting in osteoporosis. Here, we investigated the role of apolipoprotein E (ApoE), essential component of chylomicron and very low density Lipoprotein metabolic pathways, in the regulation of osteoblast and osteoclast function and thus in the pathogenesis of osteoporosis.Material and methods: We used apoE deficient (ApoE−/−) and wild ...

Introduction: Recent data suggest that lipid metabolism imbalances affect bone cell function and therefore may result in the development of osteoporosis. We investigated the role of apolipoprotein-E (ApoE), a plasma protein playing cardinal role in lipoprotein metabolism, in the regulation of osteoblast and osteoclast function and the pathogenesis of osteoporosis.Material and methods: We used apoE deficient (ApoE−/−) and C57BL/6 (c...

Fibroblast growth factor-23 (FGF23) is a bone derived phosphate-regulating hormone which is elevated in hypophosphataemic rickets. Recent findings demonstrate iron deficiency as a potential mediator of FGF23 expression and murine studies have shown in utero effects of maternal iron deficiency leading to increased FGF23 concentration and disordered bone development (Clinkenbeard. JBMR 2013). Children with rickets in rural Gambia, West Africa, have high prevalences of i...

The impact of sex and maturation on trabecular (Tb) and cortical (Ct) microarchitecture in children and young adults has not been well established. The new second-generation high-resolution peripheral quantitative CT (HR-pQCT) scanner (XCT II, Scanco Medical) incorporates three important advances to provide greater spatial resolution, direct measures of Tb thickness and spacing and measures in the Ct midshaft. The aim of this study was to identify sex and maturation effects on...

Osteocytes make up >90% of bone cells, are embedded in mineralised matrix where they form a communication network. Osteocytes differentiate from osteoblasts, and are mechano-sensitive. They are very difficult to isolate with a dependence on cell lines for in vitro studies of osteocyte biology. Therefore new methods to study these cells are essential. Recent publications indicate that osteoblasts maintained in in vitro 3D collagen gels may differentiate to...