Searchable abstracts of presentations at key conferences on calcified tissues

ba0003pp126 | Cell biology: osteoblasts and bone formation | ECTS2014

Glucose-dependent insulinotropic polypeptide directly affects collagen deposition and maturation in osteoblast cultures

Mabilleau Guillaume , Mieczkowska Aleksandra , Chappard Daniel

Objectives: A role for glucose-dependent insulinotropic polypeptide (GIP) in controlling extracellular bone matrix deposition and maturation has previously been evidenced in GIP receptor knock-out mice. However, as the GIP receptor is expressed in several tissues other than bone it was difficult to ascertain whether the modifications of extracellular bone matrix were due to direct effects on osteoblasts or indirect through regulation of signals originating from other tissues. ...

ba0003pp24 | Bone biomechanics and quality | ECTS2014

Beneficial effects of a GIP mimetic on bone material properties

Mabilleau Guillaume , Mieczkowska Aleksandra , Irwin Nigel , Flatt Peter , Chappard Daniel

Objectives: A role for glucose-dependent insulinotropic polypeptide (GIP) in controlling bone mass and strength has previously been reported. However, the rapid degradation of GIP in the bloodstream by the dipeptidyl peptidase-4 enzyme precludes therapeutic use. To circumvent this problem, a series of N-terminally modified GIP agonists have been developed. The aim of the present study was to investigate the effects of 28-day treatment with N-AcGIP on bone microarchitecture and...

ba0001oc2.5 | Bone quality and fracture repair - animal models | ECTS2013

Glucagon-like peptide 1 receptor is required for optimal bone strength and quality

Mieczkowska Aleksandra , Irwin Nigel , Flatt Peter R , Chappard Daniel , Mabilleau Guillaume

Objectives: Glucagon-like peptide 1 is secreted by intestinal L-cells into the blood supply in response to nutrients in the intestine. Although osteoblasts express the GLP-1 receptor (GLP-1R), the main action of the GLP-1/GLP-1R pathway in bone physiology and bone quality is unknown. The aim of the present study was to investigate bone strength and quality in a mouse model of GLP-1R deficiency.Materials/methods: Eight 16 weeks-old GLP-1R knock-out male m...

ba0001pp39 | Bone biomechanics and quality | ECTS2013

Glucose-dependent insulinotropic polypeptide receptor deletion results in a reduced bone strength and quality

Mieczkowska Aleksandra , Irwin Nigel , Flatt Peter R , Chappard Daniel , Mabilleau Guillaume

Objectives: Glucose-dependent insulinotropic polypeptide (GIP) is secreted by intestinal K-cells into the blood supply in response to nutrient ingestion and absorption. Although osteoblasts and osteoclasts express the GIP receptor (GIPR), the main action of the GIP/GIPR pathway in bone physiology and bone quality is unknown. The aim of the present study was to investigate bone quality in a mouse model of GIPR deficiency.Materials/methods: Eleven 16 weeks...

ba0003oc3.5 | Osteoclasts, gastric hormones and HIF | ECTS2014

Double incretin receptor knock-out (DIRKO) mice present with alterations of trabecular and cortical microarchitectures and bone strength.

Mieczkowska Aleksandra , Mansur Sity , Flatt Peter , Irwin Nigel , Chappard Daniel , Mabilleau Guillaume

Objectives: A role for the gut hormones glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) in controlling bone mass and strength has previously been reported. However, lack of one gut hormone is compensated by an elevated sensitivity to the other in single receptor knockout mice. As such the exact role of GIP and GLP-1 in bone is unclear. The aims of the present study were to assess bone mass and strength in mice with functional deletion of ...

ba0003pp100 | Cancer and bone: basic, translational and clinical | ECTS2014

Bone quality of metaplastic woven bone of mixed metastases: a FTIRI analysis

Chappard Daniel , Bouvard Beatrice , Masson Charles , Legrand Erick , Audran Maurice , Mabilleau Guillaume , Mieczkowska Aleksandra

Objectives: Most osteolytic tumors are in fact mixed and contain an osteoblastic component associated with the predominant osteolytic areas. This metaplastic woven bone can be evidenced on X-rays as blurred white areas but is always evidenced by histological analysis even in the absence of radiological expression. Metaplastic bone formation reflects the activation of new osteoblasts, the stimulation of the dormant lining cells due to the release of growth factors buried in the...

ba0003pp411 | Paediatric bone disease | ECTS2014

Tricho-rhino-phalangeal syndrome TRPS: the new three cases from Poland

Matusik Pawel , Forys-Dworniczak Elzbieta , Kalina-Faska Barbara , Januszek-Trzciakowska Aleksandra , Malecka-Tendera Ewa

Tricho-rhino-pharyngeal syndrome (TRPS) is a rare genetic disorder, which is characterized by craniofacial and skeletal abnormalities. This report presents three cases of TRPS (two sporadic and one familial). Clinical presentation included typical facial features (pear-shape nose, long flat philtrum, thin upper vermilion border and protruding ears), thin, sparse scalp hair and different skeletal abnormalities with normal mentation. Case 1: 5.5-year-old boy with short stature (...

ba0004p42 | (1) | ICCBH2015

Levels of vitamin D according to severity of motor function disorder and the level of bone mineral density in children with cerebral palsy

Zvekic-Svorcan Jelena , Nenadov Natasa , Krasnik Rastislava , Mikov Aleksandra , Vulovic Mirela , Demesi-Drljan Cila , Bekic Vera

Objective: To establish the difference between deficiency, insufficiency and normal results of vitamin D according to severity of motor function disorder and the level of bone mineral density in children with cerebral palsy.Methods: Investigation encompassed 23 children, both genders, between 6 and 17 years of age, with diagnosed cerebral palsy, who were hospitalized between January 1 and December 31, 2014. Bone mineral density (BMD) was established usin...

ba0004p43 | (1) | ICCBH2015

Efect of nutritional status on bone density in children with cerebral palsy

Krasnik Rastislava , Zvekic-Svorcan Jelena , Nenadov Natasa , Mikov Aleksandra , Demesi-Drljan Cila , Vulovic Mirela , Bekic Vera

Objective: The aim of the study was to establish effect of nutritional status on bone mineral density in children with cerebral palsy.Methods: The nutritional status was established in 23 children, both genders, with cerebral palsy, who were hospitalized at the Clinic for children habilitation and rehabilitation and at the, ‘Veternik’ Home, between January 1 and December 31, 2014. Evaluation of nutritional status has been done based on BMI. BMI...

ba0004p147 | (1) | ICCBH2015

A severe form of cerebral palsy as a risk factor for the development of secondary osteoporosis in children

Nenadov Natasa , Krasnik Rastislava , Svorcan Jelena Zvekic , Mikov Aleksandra , Drljan Cila Demesi , Radovanov Maja

Objective: Investigate bone mineral density in children with cerebral palsy (CP) with special focus on severe forms of CP.Methods: Investigation encompassed 23 children, both genders, between 6 and 17 years of age, with diagnosed cerebral palsy, who were hospitalized between January 1 and December 31, 2014. Bone mineral density (BMD) was established using Dual-energy x-ray Absorptiometry – DXA method, at L1–L4 lumbar vertebrae and at the femur ...