Bone Abstracts

Despite the improvement in bone’s molecular, metabolic and live-cell imaging, histological investigation remains most crucial in bone biology for diagnostic and research purposes. Therefore, fixation of bone samples – especially for electron microscopy investigation – is critical to the ultrastructural analysis. Up to date, chemical fixation of bone tissue is performed at room temperature resulting in a compromised ultrastructure of bone sample. In this study, w...

The healing of skeletal fractures involves a cascade of overlapping cellular events. This study aims to deepen the understanding of molecular networks orchestrating these events.Standard closed fracture in the left femur of male 8–10 weeks old C57BL/6N mice were analyzed at (day=D) D3, D7, D10, D14, D21 & D28 post fracture (N=5/time point). Total RNA was prepared for whole genome expression profiling using Illumina μ-array kit. Dat...

Purpose: The aim of our study is a micro-CT-based, longitudinal characterisation of ovariectomy, multideficient diet and corticoid therapy impact on the ossal microarchitecture.Materials and methods: Four randomized groups were formed out of 32 adult female merino land sheep:1. control2. ovariectomized (OVX)3. OVX+multideficient diet (OVX-D)4. OVX-D+320 mg methylp...

FDA guidelines recommend the sheep as large animal model for osteoporosis. Studies of osteoporosis induction use dual energy x-ray absorptiometry (DEXA) to evaluate bone status. Experimentally, the T-score is calculated based on reference bone mineral density (BMD) values of animals before treatment (0 M). Whereas clinically the reference BMD represents values of an independent group of patients around 30 years old. The study hypothesize that the use of additional ind...

Objective: Oosteoporoti fractures are a growing problem especially in aged societies of industrialized countries. Therefore, a clinical demand for synthetic bone graft substitutes is increasing. Despite the general success CPC showed clinically, development of CPC-based material by adding stronsium could improve its suitability to treat osteoporotic fractures.Methods: Sprague–Dawley rates model of induced osteoporosis via multi-deficiencies diet com...

Due to its huge socio-economic impact a better understating of osteoporotic fracture healing is crucial.Thirty-one female merino land sheep were randomly divided into four groups. (i) Untreated control-group (C, n=8); (ii) bilateral ovariectomy (OVX, n=7); (iii) OVX and calcium-deficient diet (OVXD, n=8); and (iv) OVXD and additional biweekly corticosteroid injections (OVXDS, n=8). Drill-hole defects (7.5 mm in diamete...

Interference fit fixation of soft-tissue grafts is in clinical focus to allow anatomic graft fixation which increases stability and graft isometry. Although clinical data show promising results with different screw materials, the issue of biodegradability, handling and stability in the first weeks remain under demand. The purpose of the present study was to investigate anterior cruciate ligament (ACL) reconstruction radiologically, biomechanically and histologically using dire...

Neurofibromatosis type I (NF1) is a monogenetic disorder caused by mutations in the NF1 gene encoding for the Ras-GAP protein neurofibromin. Apart from benign tumour development NF1 is frequently associated with skeletal manifestations such as osteopenia or debilitating focal skeletal dysplasia. To assess a function of Nf1 in osteocytes we here apply a combinatorial approach of biophysical, histological and molecular techniques allowing differential analysis ...

Introduction: Vitamin D is essential for calcium metabolism as well as for fracture prevention. In children, low levels can cause rickets. A high prevalence of inadequacy in children has been reported in many studies. It is entirely prevalent in Lebanese children. There are different ways to detect cut-off points for vitamin D deficiency, The aim of this study was to describe the distribution of serum 25(OH)D levels in Lebanese children, males and females, to evaluate and prop...

Aim: The aim of this study was to assess the calcium daily food intake in Lebanese women living in South Lebanon, an under privileged area.Subjects and methods: Assessment of calcium food intake was performed with a validated questionnaire in 290 female patients attending clinics in two main referral health centers in South Lebanon.Results: The mean age of patients was 54.76 years and the mean BMI was 30.07 kg/sqm (S.D.</sma...