Bone Abstracts

1,25-Dihydroxyvitamin D (1,25D) is one of the major regulators of calcium metabolism. Due to its lipophilic nature and low circulating concentration, the measurement of 1,25D concentration levels has been labour intensive and technique dependent in addition to multiple equipments required for the sample purification procedure. We reported the results of soon to be commercialised fully on-board IDS-iSYS 1,25 VitDXp assay.IDS-iSYS 1,25 VitD...

Objectives: The Brtl mouse, a unique model for the autosomal dominant forms of osteogenesis imperfecta was used to prove the feasibility of a lentiviral-shRNA-based strategy to improve collagen quality by targeting the mutant Col1a1 allele at the point mutation responsible for the causative substitution Gly349Cys. The ability to specifically suppress the mutant allele should convert the moderate Brtl outcome to the mild one caused by quantitative defect.<p class="...

Small GTPases of the Rho family (RhoA and Rac-1) are responsible for cytoskeleton dynamics (particularly actin polymerisation) and control cellular tension. For these reasons, they are implicated in the commitment of multipotent cells (MCs). In one hand, increased tension (important RhoA activity) is commonly associated with osteogenesis (OS), in the other hand, a reduced one (low RhoA activity) is associated with adipogenesis (AD). Nevertheless, precise RhoGTPases regulations...

Nacre, or mother of pearl, is a promising natural biocompatible biomaterial consisting of aragonite (97%) and of organics (3%) and capable to increase the cell osteogenic activity. It has been established that osteoarthritic osteoblasts present a mineralization defect and, to date, only a few molecules (vitamin D3 and bone morphogenetic protein2) could improve the mineralization potential of this cell type. In this context, we evaluated the impact of nacreous molecules on the ...

Nacre is able to induce bone-forming cells mineralization, and gains widespread interest in bone regeneration. While, the osteoinductive compounds are not identified yet. The nacre extract, ethanol soluble matrix (ESM), was proven having the capacity to induce the mineralization of mouse MC3T3-E1 cells and to restore mineralization defect in human osteoarthritic osteoblasts. Being tested on the two cells, ESM could induce the formation of mineralization nodules, identified as ...

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Bone-marrow mesenchymal stem cells (MSCs) are the origin of bone-forming cells with immunomodulation potential. Among the generated immunosuppressive molecules there is HLA-G5. HLA-G proteins play a crucial role in promoting the acceptance of allografts. However, the mechanisms regulating the expression of HLA-G5 in human MSCs are unknown. We induced differentiation of human MSCs (harvested from iliac crests of healthy volunteers after their informed consent following approved...

Rational: Mortality due to non-small cell lung cancers is the first cause of cancer death in men around the world. Lung adenocarcinoma regularly induces bone metastases responsible for high morbidity and impaired life quality. Overall survival of these patients is poor. Thus we aimed to identify if some bone and metabolic parameters were associated with overall survival.Patients and Methods: POUMOS is a prospective cohort of patients suffering from adeno...

Background: Hypocalcemia presenting in adolescence is rare. Most common etiology is autoimmune hypoparathyroidism. Pseudohypoparathyroidism (PHP) is a rare group of disorders characterized by end-organ resistance to parathyroid hormone (PTH), and other hormones, such as TSH, with or without features of Albright’s hereditary osteodystrophy.Case presentation: A 14-year-old boy presented complaining of fatigue and spontaneous carpal spasms in associati...