Bone Abstracts

Being efficient and productive in research is frequently linked to a structured organization of the available research time. This applies to any individual scientist including PhD students, Postdocs, or PIs. In particular PIs but also to some extend more senior Postdocs have a responsibility for more junior scientists and in the case of PIs even of an entire research team. This does not only require overseeing and organizing his own time but also the time and workload of other...

Bone is a highly dynamic tissue that is constantly dismantled and rebuilt throughout life by the coordinated and balanced activities of matrix-resorbing osteoclasts, bone-forming osteoblasts and matrix-embedded osteocytes. This system is governed by interconnected signaling pathways, transcription factors, secreted regulators, genetic determinants and epigenetic cues including non-coding RNAs. Canonical Wnt signaling is among the most influential pathways regulating tissue dev...

Osteosarcoma is the most prevalent primary bone tumor that occurs mainly during adolescence. Osteosarcoma is an aggressive mesenchymal tumor that often arises due to mutations in the tumor suppressor gene p53. Treatment of osteosarcoma patients includes chemotherapy, radiation therapy and surgical approaches. Nevertheless, at advanced stages the survival rate is rather low. Thus, a better understanding of the underlying biology of osteosarcoma might help to develop novel thera...

Biographical DetailsEric HesseEric Hesse studied Medicine at Hannover Medical School in Germany where he became MD in 2003. He was trained in Orthopedic Surgery and graduated as PhD in 2007 in Genetics & Cell Biology in Hannover, Germany. In 2005, he moved as a Postdoctoral Fellow funded by the German Research Foundation to the laboratory of Dr ...

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Senile osteoporosis and age-related osteopenia are associated with decreased osteoblastogenesis and increased bone marrow adipogenesis. The mechanisms controlling the fate determination of osteoblast to adipocyte differentiation of bone marrow stromal cells (BMSC) during aging are not known. We and others previously showed that the cell-cell adhesion molecule N-cadherin (N-Cadh) expressed in osteoblasts controls bone formation, but little is known about its role in BMSC fate d...

Purpose: Osteoclastogenesis is enhanced in osteoarthritis (OA). We have demonstrated that cartilage breakdown is reduced when osteoclastogenesis is inhibited in murine models. Wnt activity, known to regulate the bone and chondrocyte cell function, might contribute to the mechanisms that promote cartilage catabolism. Our purpose was to evaluate whether osteoclast-secreted molecules affect the chondrocyte metabolism and assess the contribution of Wnt pathway.<p class="abstex...

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Objective: Wnt/β-catenin pathway is a main regulator of bone remodeling, but might be inhibited in cartilage in osteoarthritis (OA). We here investigated the effect of mechanical loading in Wnt activation and the expression of Wnt antagonists in the joint tissues.Methods: Topgal mice were used. Mice underwent partial meniscectomy (Mnx) and sacrificed at 4, 6, and 9 weeks. Dissected knees were scanned by microCT and then prepared for cryosectioning t...

Aim: Wnt/β-catenin pathway promotes cartilage breakdown in osteoarthritis. We have previously shown that sclerostin preserves chondrocyte maintenance in vitro by reducing chondrocyte catabolism through the inhibition of Wnt/β-catenin pathway. However, sclerostin restores partially the expression of the anabolic genes. We therefore investigated the effect of sclerostin in the activation of Wnt non canonical pathways mediated by Ca2+/CaMKII, JNK, an...