Bone Abstracts

see PP149....

The molecular mechanisms underlying telomere shortening and telomerase deficiency contributing to defective age-related bone formation are poorly studied. We have previously demonstrated the role of telomerase re-activation in enhancing osteoblast differentiation of BMSCs in vitro and in vivo. Here, we investigated further the signaling pathways underlying the regulatory function of telomerase in osteogenesis. Comparative microarray analysis of telomerase-ove...

see P146....

Identifying novel approaches for enhancing osteoblast (OB) differentiation of human skeletal (mesenchymal) stem cells (hMSC) can lead to development of novel anabolic agents required for efficient bone formation. Transforming growth factor-βs (TGF-β1, 2, 3) are one of the most abundant growth factors in bone and play a key role in regulating bone remodeling. Canonical TGF-β signaling inhibits, whereas components of the non-canonical TGF-β signaling (e.g. Ak...

Objective: To comparing the role of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) in fat and bone communication.Methods: Male wild type (WT) mice, IL-6 knockout (IL-6−/−) mice, and tumor necrosis factor alpha (TNF-α) were fed with either standard diet (SD) or high fat diet (HFD) for 12 weeks. Bone mass and bone microstructure were evaluated by micro-CT. Gene expression related to lipid and bone metabolisms ...

see PP175....

Identifying novel molecules that enhance human skeletal (mesenchymal) stem cells (hMSC) differentiation into osteoblastic bone forming cells (OB), may lead to development of new bone anabolic drugs. We have identified Kix, a small molecule kinase inhibitor that enhanced ex vivo OB differentiation and reduced apoptosis of hMSC. We found that Kix targeted undifferentiated hMSC populations and not their differentiated progeny. In addition, Kix increased in vivo ...

We found that miR-335 is reduced in SBC-5, a human lung cancer (LC) derived cell lines inducing osteoclast formation by expressing RANKL; reviving miR-335 expression in SBC-5 results in decrease of its proliferation, invasion, migration and clone formation in vitro; reviving miR-335 expression in LC model leads to reduction of bone metastasis (BM) in vivo. It was confirmed that miR-335, inhibiting the formation and activity of osteoclasts and reducing the pro...

Background: Hypoxia induces mesenchymal stem cells (MSCs) proliferation but results in a population with impaired osteogenic differentiation potential. Bone healing of tibial lengthening is enhanced by hyperbaric oxygen therapy. However, little is known about the effects of HBO on the Wnt signaling pathway in MSCs.Materials and methods: MSCs were cultured in complete medium and the osteogenic groups were cultured in osteogenic induction medium. Control c...

Background: Autocrine and paracrine Wnt signaling operates in stem cell populations and regulates mesenchymal lineage specification. In Wnt producing cells, Wntless (GPR177) supports the transport of Wnt from the trans-Golgi network (TGN) to the cell surface in vesicles, from which Wnt is then released. The retromer complex (VPS35) interacts with Wntless and retrieves Wntless from endosomes back to TGN, thereby maintaining the normal levels of Wntless protein. V-ATPases-driven...