Bone Abstracts

Osteoblasts produce protein-crosslinking enzymes, transglutaminase 2 (TG2) and factor XIIIA (FXIIIA), which regulate fibronectin matrix stabilization and osteoblast differentiation in vitro. To examine if they are important in bone remodeling and in maintenance of bone quality and mass in vivo, we performed skeletal phenotyping of Tgm2−/− and F13a1−/− mice and generated a double-null Tgm2</em...

The development of optimally performing biomaterials mimicking the natural physiological processes underlying bone repair is essential for non-healing large bone lesions. We sought to develop scaffolds that fill the lesions and improve bone healing. We tested three scaffolds comprised of varying percentages of lactide (LA), caprolactone (CL), and methacrylate produced by two-photon photopolymerization; LCM 3 (8:2; 85%), 4 (9:1; 90%), and 6 (9:1; 40%). We hypothesized that the ...

Introduction: Granulocyte colony-stimulating factor (G-CSF) is widely used to mobilize peripheral blood stem cells (PBSC) and enable PBSC collection by apheresis. Although bone pain is a common adverse event following G-CSF treatment, little is known on its effect on bone metabolism.Methods: Markers of bone turnover (OC, osteocalcin, β-CTx, bALP, C-terminal telopeptide of type I collagen, bone specific alkaline phosphatase, TRAP, tartrate resistant ...

Non-healing fractures caused by trauma, disease or tumour resection demand the use of bone grafts to support and stimulate the healing process. We sought to develop and evaluate the effect of a novel bioactive biodegradable biomaterial designed to fill large bone lesions and to improve bone healing. We used two-photon polymerised synthetic polymer scaffolds composed of lactide (LA), caprolactone (CL), and methacrylate (MA) with varying LA: CL ratio and percentage of methacryla...

Osteogenesis imperfecta (OI) is a genetic disorder, leading to fragility of the bones. The clinical variability is extreme, ranging from relatively mild to perinatally lethal. Secondary features such as short stature, blue sclerae, dentinogenesis imperfecta and hearing loss may also exist in affected individuals. OI is most often caused by mutations in the collagen type I genes COL1A1 and COL1A2, that show a dominant mode of inheritance. The least severe OI c...

Biographical DetailsG Pals is Director of the Centre for Connective Tissue Research at the VU University Medical Center (VUMC) in Amsterdam, The Netherlands. Following completion of his MSc in Biochemistry in Utrecht he completed his PhD training in Human Genetics in Amsterdam. In 1987 he held a position as Visiting Scientist/Professor at Wayne State University in Detroit, USA, and then a...

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Glucocorticoids (GCs) are widely used to treat chronic inflammatory diseases such as rheumatoid (RA) and lead to multiple side effects including glucocorticoid induced osteoporosis (GIO). Our work challenges the dogma that transrepression of pro-inflammatory genes by the glucocorticoid receptor (GR) is solely responsible for reducing inflammation, whereas transactivation of genes is causing side effects.Using conditional and function selective mutant mic...

Objectives: This cross-sectional study was done in order to assess the prevalence and determinants of vitamin D deficiency in children taking in account the following parameters: children’s age, season, geographic region and ethnicity.Methods: A cross-sectional prospective study was conducted in Israeli children aged 0–18 from Meuhedet Health Services during the year of 2016 in primary care pediatric clinics throughout all different geographic ...

Fibrodysplasia ossificans progressiva (FOP) is a rare, extremely disabling genetic disorder characterized by progressive heterotopic ossification preceded by episodic inflammatory soft tissue swellings (flare ups) leading to early death. There is no proven effective treatment yet. We aimed to develop an in vitro system to investigate the working mechanism of flare ups induced ossification.Skin biopsies were obtained from four patients with FOP. ...