Bone Abstracts

The oim/oim mouse resembles type III OI. Our study mimics clinical questions: is RANK-Fc as efficacious as ALN in reducing fracture number? Is RANK-Fc safe to administer to children? Is there any advantage to receiving RANK-Fc treatment post adolescence, following no treatment or ALN treatment from infancy through adolescence?All animal work was done under an IACUC-approval. Mice – n=200, WT or oim/oim. Treatment spanned a...

Objective: The purpose of this study was to determine whether osteogenesis imperfecta (OI) patients entering adulthood should continue with bisphosphonate therapy or would benefit from switching to a RANKL blockade therapy. To address this question, we used a mouse model of type III OI.Methods: Animal studies were performed under IACUC approval. OI (oim/oim) and wild-type (WT) mice were treated from 2–26 weeks with i) saline; ii) alendronat...

Objective: The purpose of this ongoing study was to determine whether osteogenesis imperfecta (OI) patients entering adulthood should continue with bisphosphonate therapy or would benefit from switching to a RANKL blockade therapy. To address this question, we used a mouse model of type-III OI.Methods: Under IACUC-approval, +/+ and oim/oim mice were treated from 2–26 weeks (n=20/treatment/genotype) with either 1) saline 24 weeks; 2...

Vertebral fracture assessment (VFA) by DXA is an accepted tool in adults. However, its use in children has not been validated. The aim of this study was to validate VFA using iDXA against spinal radiographic assessment (RA) for the identification of vertebral fractures in children.Spine radiographs and VFA (L5–T2) by GE-iDXA were acquired on the same day in 80 children. Forty children were considered high-risk for fracture as their metabolic bone sp...

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Introduction: Fracture risk assessment using FRAX® estimates 10-year fracture risk (FR10) at major sites (Maj_FR10) and hip (Hip_FR10). In 2012, in Newcastle, UK, a strategy was agreed to share data between General Practice (GP), Clinical Commissioning Groups and local hospitals to identify patients at high FR10.Methods/design: FRAX® and RAID® (a health reporting, analysis and intelligence delive...

Background: Autocrine and paracrine Wnt signaling operates in stem cell populations and regulates mesenchymal lineage specification. In Wnt producing cells, Wntless (GPR177) supports the transport of Wnt from the trans-Golgi network (TGN) to the cell surface in vesicles, from which Wnt is then released. The retromer complex (VPS35) interacts with Wntless and retrieves Wntless from endosomes back to TGN, thereby maintaining the normal levels of Wntless protein. V-ATPases-driven...

Background: The activation of Wnt/β-catenin signaling promotes cellular senescence and induces matrix metalloproteinases (MMPs) expression in intervertebral disc (IVD). However, little is known about the effects of hyperbaric oxygen (HBO) on the Wnt/β-catenin signaling and MMPs expression in degenerated human IVDs.Materials and methods: Nucleus pulposus cells (NPCs) were separated from the degenerated disc nucleus tissue by performing sequentia...

Background: Expression of toll-like receptor (TLR) 2 and 4 was up-regulated in lesional areas of OA cartilage. Hyperbaric oxygen (HBO) reduces the TLR signalling pathway in multiple organ failures. However, little is known about the effects of HBO on the catabolic pathway mediated by TLRs in human osteoarthritic (OA) chondrocytes.Materials and methods: OA chondrocytes were separated from the knees of OA patients by performing sequential enzymatic digesti...

Background: Hypoxia induces mesenchymal stem cells (MSCs) proliferation but results in a population with impaired osteogenic differentiation potential. Bone healing of tibial lengthening is enhanced by hyperbaric oxygen therapy. However, little is known about the effects of HBO on the Wnt signaling pathway in MSCs.Materials and methods: MSCs were cultured in complete medium and the osteogenic groups were cultured in osteogenic induction medium. Control c...