Bone Abstracts

Primary hyperparathyroidism is extremely rare in childhood and adolescence. Here we report a clinical case of a 15-year-3-month-old boy who began 2 years before with pain in his knees, genu varum, and fatigue. Physical examination showed severe genu valgum with an inter-malleolar distance of more than 30 cm. Biochemical tests showed hypercalcemia (12.2 mg/dl), hypophosphatemia (2.3 mg/ dl), hypercalciuria (6.4 mg/kg per day), high alkaline phosphatase (2812 IU/l), low 25-hydro...

Mammalian target of rapamycin (mTOR) functions mainly in the form of two complexes, namely mTORC1 and mTORC2, which are distinct in their unique components, raptor and rictor. Here, we focused on bone phenotypes in mice with a specific deletion of rictor using a Cre recombinase gene whose expression was driven by the promoter of osteocalcin. All procedures involving mice were approved by the Institutional Animal Care and Use Committee of the local admin. DXA analysis showed a ...

Mammalian target of rapamycin (mTOR) functions mainly in the form of two complexes, namely mTORC1 and mTORC2, which are distinct in their unique components, raptor and rictor. Here, we focused on bone phenotypes in mice with a specific deletion of rictor using a Cre recombinase gene whose expression was driven by the promoter of osteocalcin. All procedures involving mice were approved by the Institutional Animal Care and Use Committee of the local admin. DXA analysis showed a ...

The purpose of this study was evaluated the osteogenic differentiation in vitro of mesenchymal stem cells (MSC) of SHR (Spontaneously Hypertensive Rats) and Wistar (Normotensive) rats.Therefore, MSC from femoral bone marrow of Wistar and SHR rats 4 weeks old were utilized. The experimental protocols were approved by the Animal Experiments Local Ethics Committee (Process 00716-2012). The osteogenic medium (OM) consist of the proliferation medium (MEM) supplemented with...

The aim of this study was to investigate the biocompatibility of hydroxyapatite (HAP) powder from whitemouth croaker fish (Micropogonias furnieri). For this purpose, fragments from HAP with 0.5 cm2 were inserted in the subcutaneous tissue of animals. After 7, 15, and 30 days, histopathological analysis was performed. The results showed that it was possible to detect tissue reactions closely related to cytotoxicity in a time-exposure manner. At day 7, modera...

In this study, the effect of food restriction of 30% in calorie intake on femurs of rats of different ages was investigated. Male Rattus novegicus albinus rats, Holtzman lineage, aged 38 days, 4 and 16 months were used. The animals were divided into six groups (n=8–10 per group), three being control groups (C38, C4 and C16) and three groups on restricted diet (R38, R4 on R16). The restricted animals were submitted to food restriction of 30% being fed wit...

Fracture prevalence in children appears to change in recent years due to variations in physical activity and enforcement of laws that protect children within motor vehicles. However, in Mexico such variation has not been explored so far.Objective: To analyze fracture prevalence in the paediatric population of Mexico to detect patterns of change in time, between genres and among different diagnoses.Methods: We analyzed data from the...

Osteoporosis is a chronic disease with a high prevalence in older adults. There is evidence that statins have some beneficial effects on bone metabolism. Although the trial reports are ambiguous, it is now accepted that statins block the osteoclast differentiation inhibiting to some extent bone resorption. Our aim was to evaluate the effects of a statin in an animal model, which was induced to osteoporosis by glucocorticoids and then treated with atorvastatin. The experimental...

It is widely accepted that the “gold” standard method for diagnosis of osteoporosis is dual energy X-ray absorptiometry, to measure bone mineral content and bone mineral density (BMD). However in the last decades other less harmful and cheaper methods were developed. Among them bioelectrical impedance analysis (BIA) is one of most commonly used and is used in analysis of body composition as well. On its side, quantitative ultrasound (QUS) is a noninvasive method of e...

Vitamin D plays an important role for bone health, and is associated to the risk of osteoporosis development.The aim of the present work was to determine the association between serum vitamin D concentration and the bone mineral density (BMD) in post-menopausal women living in a Mexican rural community (this study was approved by the Institutional Ethics Committee). The BMD was assessed by ultrasound and vitamin D was evaluated using a specific immunoass...