Bone Abstracts

Introduction: Osteoporosis is frequently associated with renal disease, namely the bone metabolism disturbances caused by secondary hyperparathyroidism of chronic kidney disease (CKD). The increased risk of fragility fractures is well demonstrated in patients with end-stage renal disease (ESRD). There is recent evidence that bone pathological changes start early in the course of CKD. Our aim is to evaluate whether chronic renal disease, before ESRD, is associated with bone fra...

Background: Zoledronate, a third generation bisphosphonate, has showed high efficacy in the inhibition of bone resorption. The objective of this observational study was to assess short and long-term efficacy and safety of zoledronate in the treatment of active Paget’s disease (PD).Methods: Patients with active PD treated with zoledronate 5 mg were consecutively recruited. Clinical and laboratory parameters were determined before, at 3 and every 6 mo...

Bone tissue engineering relies on the combination of scaffolds and cells aiming to heal bone defects. Among the many candidates for scaffolds, gelatin sponge (Gelfoam®) and fibrin glue (Tissucol®) are of interest due to the well-established biocompatibility and the ability to be loaded with cells. Here, we evaluated their potential to stimulate bone formation by microtomography analysis. For this, 5-mm unilateral calvarial defect was created in rats deeply anesthetiz...

Background: We have previously found in the chronic SKG mouse model of arthritis that long standing (5 and 8 months) inflammation directly leads to high collagen bone turnover, disorganization of the collagen network, disturbed bone microstructure and ultimately declining in bone biomechanical properties. Our main goal was to study the effects of the inflammatory process on the microarchitecture and mechanical properties of bone in the early stages of arthritis development.</p...

Titanium (Ti) surface with nanotopography induces osteoblast differentiation, at least in part, by upregulating the expression of α1/β1 integrins. Thus, we hypothesized that other potential candidates from the integrin signalling pathway may be involved in the osteogenic potential of nanotopography. In this context, the aim of this study was to compare the expression of integrin family members of cells grown on nanotopography with machined surface. Machined Ti discs ...

In this study, we evaluated the effect of the association of a bioactive glass-ceramic scaffold (Biosca) with mesenchymal stem cells derived from either bone marrow (BM-MSCs) or adipose tissue (AT-MSCs) on bone formation in calvarial defects of osteoporotic rats. Wistar rats were submitted to bilateral ovariectomy (OVX) or only to the surgical stress (Sham), under approval of the Committee of Ethics in Animal Research. After 5 months, 5-mm unilateral calvarial defect was creat...

Osteoclasts are multinucleated giant cells derived from the monocyte/macrophage lineage in the presence of receptor activator of nuclear factor kappa-B ligand (RANKL) and colony-stimulating factor 1 (CSF1). The bone remodeling process in zebrafish is incompletely understood. Here we describe several methods to quantify bone formation and resorption using a zebrafish mutant that lacks functional colony stimulating factor 1a receptor (csf1ramh5/mh5). Mice deficient in...

Osteoclasts are myeloid-derived cells that degrade bone through the localized production of acid and proteases. The catabolic action of osteoclasts is counterbalanced by formation of new matrix by osteoblasts. In mammals, bone resorption and formation are coupled through crosstalk between osteoclasts and osteoblasts. Zebrafish are emerging as a valuable model to study bone biology, but how closely zebrafish osteoclast function parallels that of higher vertebrates is unknown. T...

Background: Autosomal dominant hypocalcaemia (ADH) is caused by activating mutations of the calcium sensing receptor (CaSR). Symptomatology ranges from asymptomatic hypocalcaemia to paraesthesia, tetani, laryngospasm and seizures. This is the first report of congenital hyperinsulinism (CHI) in a child with ADH.Presenting problem and clinical management: A female infant, born at term from non-consanguineous parents. She presented on D2 with persistent asy...

Rheumatoid arthritis (RA) and ankylosing spondylitis (AS) are systemic, immune-mediated diseases. RA’s main targets are the peripheral joints while AS has the axial skeleton and enthesis as the principal affected areas. RA is characterized by bone erosions and impaired repair whilst AS is typified by bone overgrowth. The causes for these differences are not yet understood; however we hypothesize that AS patients’ monocytes receive reduced osteoclastogenic stimuli and...