ECTS2016 Poster Presentations Bone development/growth and fracture repair (35 abstracts)
The effects of combined teriparatide and denosumab on bone regeneration
Kazuma Kitaguchi , Masafumi Kashii , Tsuyoshi Sugiura , Morimoto Tokimitsu , Takaaki Noguchi , Takahiro Makino , Kosuke Ebina , Takashi Kaito & Hideki Yoshikawa
Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
Objective: The purpose of this study is to investigate the effects of the combined teriparatide (TPTD) and denosumab (DMAB) on both cortical and cancellous bone regeneration.
Materials and methods: Bone defects were created in the diaphysis of the right femur and in the epimetaphysis of the left femur in 8-week old female C57/BL6N mice. After making bone defects, Mice were given either saline or 40 μg/kg TPTD for 20 days (5× per week) and were simultaneously given either single-shot saline or 5 mg/kg DMAB. The following assessments were performed in the four groups (Control vs TPTD vs DMAB vs COMB): time-course bone microstructural analysis of both cortical and cancellous bone defects, with in vivo microcomputed tomography (μCT); histological and biomechanical analysis of both cortical and cancellous bone defects after euthanasia.
Result: In the epimetaphysis, the bone defect was dramatically healed over time in the COMB group, and the bone mass of newly formed cancellous bone in the COMB group significantly increased compared to the other 3 groups 15 days postoperatively (Control: 0.01, TPTD: 0.12, DMAB: 0.17, COMB: 0.34; P<0.05, unit: mm3). In the diaphysis, there were no significant differences in the bone mass of newly formed cortical bone among four groups over time. Histologically, many woven bones and cartilage matrix still existed and lamellar bone was not well formed in the both COMB and DMAB group.
Conclusion: These results suggest that combined TPTD and DMAB promotes the bone healing in the cancellous bone rich area like spine.
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Bone Abstracts
ISSN 2052-1219 (online)
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