ECTS2016 Poster Presentations Osteoporosis: treatment (40 abstracts)
Lithium chloride enhances bone formation and implant osseointegration in osteoporotic condition
Yifan Jin 1 , Hualin Zhang Zhang 2 , Xiaohui Hu 2 , Gang Wu 3 , Yiwen Dong 1 , Zhennan Deng 1 , Shengbin Huang 1 , Janak L Pathak 4 & Jinsong Liu 1
1School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China; 2College of Stomatology, Ningxia Medical University, Yinchuan, China; 3Department of Oral Implantology and Prosthetic Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, MOVE Research Institute Amsterdam, Amsterdam, The Netherlands; 4Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology (SPST), Tianjin University, Tianjin, China.
Osteoporotic patients have poor bone quality and high risk of dental and orthopaedic implant failure. Lithium chloride (LICl) has been reported to enhance osteogenesis. However, the role of LiCl in bone formation and implant osseointegration in osteoporotic condition is still unknown. Therefore, we aimed to investigate the effect of LiCl on bone formation and implant osseointegration in osteoporotic rats.
Three months old Sprague–Dawley female rats (n=27) were randomly divided into two groups and performed ovariectomy or sham surgery. After 3 months of surgery, titanium implants were implanted in tibial medullary canal. After implantation, ovariectomized rats were treated with 150 mg/kg per 2 days of LiCl (ovariectomy+LiCl) or saline, and sham-operated rats with saline by oral gavage for 3 months. Tibias with implants were harvested. Bone formation and implant osseointegration was analyzed by using histology, micro-CT, biomechanical testing, and immunohistochemistry.
Histological images clearly showed decreased number and distribution of trabeculae in tibia of ovariectomized rats. Interestingly, ovariectomy+LiCl increased number and distribution of trabeculae in tibia. Micro-CT data showed that in ovariectomy reduced bone volume tissue volume ratio (BV/TV) by 3.6-fold, trabecular number (Tb.N) by 1.8-fold, trabecular thickness (Tb.Th) by 1.6-fold, and increased trabecular space (Tb.Sp) by 2.2-fold. Ovariectomy+LiCl increased BV/TV by 1.9-fold, Tb.N by 1.4-fold, Tb.Th by 1.3-fold, and decreased Tb.Sp by 1.2-fold. Bone histology and biomechanical testing showed that ovariectomy reduced bone implant contact (BIC) by 5.2-fold, implant osseointegration by 5.4-fold, and implant mechanical fixation by 5.5-fold. Ovariectomy+LiCl increased BIC by 4.4-fold, implant osseointegration by 3.3-fold, and implant mechanical fixation by 2.9-fold. Ovariectomy+LiCl increased β-catenin expression by bone cells around the implants.
In conclusion, our study shows that LiCl enhances bone formation and implant osseointegration in osteoporotic rats, suggesting LiCl as a promising therapeutic agent to increase bone mass as well as to prevent implant failure in osteoporotic condition.
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