ECTS2016 Poster Presentations Osteoporosis: pathophysiology and epidemiology (55 abstracts)
The role of melatonin in regulating autophagy in type 2 diabetic osteoporosis
Weilin Zhang , Hongzheng Meng , Maowei Yang , Guanghong Sun , Junhua Liu , Pengxu Shi , Fei Liu & Bo Yang
Department of Orthopedics, The First Hospital of China Medical University, Shenyang, China.
Objective: The study of melatonin on the levels of autophagy in bone will contribute to new type 2 diabetes osteoporosis-pathological processes by which a specific role and mechanism for the treatment of type 2 diabetes-induced osteoporosis could emerge.
Method: This study consists of a two-part experiment. We assessed different body parameters in rats, a type 2 diabetes model application, utilizing different concentrations of melatonin interventions, the application of dynamic biomechanical measurements, bone organization hard slice dyeing, and micro-CT, which are all methods for rat bone micro-structure observation. We also applied immunohistochemistry method of rat bone organization within autophagy level observation. We also performed in vitro experiments on hFOB1.19 cells cultured with high glucose, different concentrations of melatonin and ERK pathway inhibitors and then performed western blotting and immunofluorescence on cells to assess their osteogenic ability and levels of autophagy.
Results: (1) Melatonin could significantly improve the bone microstructure of our rat model of type 2 diabetes and reduce the levels of autophagy. 100 mg/kg is better than 50 mg/kg. (2) Melatonin could improve the osteogenic capacity of osteoblasts at high glucose levels and reduce the levels of autophagy in osteoblasts cultured at high glucose levels. 10 μM is better than 1 mM.
Conclusion: The proper concentration of melatonin can inhibit the ERK signalling pathway and thus reduce the levels of autophagy in osteoblasts as well as delay type 2 diabetes-induced osteoporosis.
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Bone Abstracts
ISSN 2052-1219 (online)
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