ECTS2016 Poster Presentations Genetics and Epigenetics (25 abstracts)
Association among oxidative stress, Wnt signaling and trabecular bone microstructure in osteoporosis and osteoarthritis
Mercè Giner 1, , Cristina Miranda 1 , M. José Montoya 2 , Sergio Portal 3 , M. Angeles Vazquez 2 , M. José Miranda 1 , Pedro Esbrit 3 & Ramón Perez-Cano 1,
1HUV Macarena, Seville, Spain; 2University of Seville, Seville, Spain; 3Fundación Jiménez Diaz, Madrid, Spain.
Experimental studies suggested that both, oxidative stress and the Wnt pathway, are important factors in the regulation of bone remodeling. Thus, low antioxidant levels and elevated markers of Wnt pathway inhibitors (sclerostin) levels are associated with a reduced bone mineral density and increased risk of osteoporotic fracture. Whether oxidative stress and the Wnt pathway are related to fracture risk is poorly understood.
M&M: Cross-sectional study in 21 subjects divided into three groups: seven osteoporotic hip fracture (age: 75±5) (OP); eight osteoarthritis, undergoing hip replacement, (71±4) (OA) and six OA ≤ 55 years old.
We carried out hip BMD (DXA-Hologic Discovery) and microstructural and biomechanical characteristics of trabecular bone (Micro-CT-Scan Sky 1172). In macerated trabecular bone, we quantified gene expression of catalase, GADD45 (oxidative stress genes), connexin 43, cyclin D1 (Wnt pathway genes), Runx2, osteoprotegerin (OPG) and sclerostin (SOST) by qPCR.
The results are analyzed statistically with the Kruskal–Wallis and Dunn’s post hoc and correlations by Pearson coefficient (SPSS 22.0), P≤ 0.05.
Results: Osteoporotic subjects have an increased expression of catalase and GADD45 in trabecular bone, suggesting increased oxidative stress in these patients regardless of age and sex.
We also observed a significant increase in the expression of genes involved in the Wnt pathway, connexin 43 and cyclin D1, Runx2 and OPG, in OP group. We found no differences in the SOST gene expression.
As expected, BMD values are statistically lower in the OP subjects and they have a worse biomechanic and microstructure bone.
Conclusion: These results suggest that the trabecular bone from patients with osteoporosis have a higher activity of oxidative stress and alterations in the Wnt pathway and osteogenic genes expression.
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Bone Abstracts
ISSN 2052-1219 (online)
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