ECTS2016 Poster Presentations Cancer and bone: basic, translational and clinical (37 abstracts)
Polycystin-1 is involved in osteosarcoma pathobiology
Christos Adamopoulos 1 , Antonios N. Gargalionis 1 , Lina Malakou 1 , Maira Katsianou 1 , Christofora Grivaki 1 , Georgios Agrogiannis 2 , Penelope Korkolopoulou 2 , Christina Piperi 1 , Efthimia K. Basdra 1 & Athanasios G. Papavasiliou 1
1Department of Biological Chemistry, University of Athens Medical School, Athens, Greece; 2First Department of Pathology, University of Athens Medical School, Athens, Greece.
Osteosarcoma is the most common primary bone-derived tumor. However, the pathogenic molecular mechanism(s) underpinning osteosarcoma development and metastasis remain elusive. The polycystins PC1 (polycystin-1; encoded by PKD1) and PC2 (polycystin-2) are central players in mechanotransduction, a process that can influence all steps of the invasion/metastasis cascade. Recent studies from our laboratory provided, for the first time, a direct link between mechanosensing polycystins and colorectal cancer.
Our aim was to investigate the potential role of PC1 in osteosarcoma pathogenesis.
Immunohistochemical expression of PC1 was evaluated in human paraffin-embedded osteosarcoma tissues. MG-63 osteosarcoma cell line was cultured in vitro and functional assays were performed following PC1 extracellular inhibition in order to assess cell proliferation and migration. PKD1 knockdown was achieved in MG-63 cells transfected with PKD1-specific siRNA.
PC1 presented basic levels of endogenous protein expression and predominant nuclear localization in osteosarcoma tissues. Functional inhibition of PC1 was associated with increased cell proliferation and migration in MG-63 cells. PKD1 knockdown was accompanied by activation of key components of the Wnt and PI3K/Akt/mTOR signaling pathways.
PC1 is implicated in the molecular mechanism(s) underlying osteosarcoma pathobiology.
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Bone Abstracts
ISSN 2052-1219 (online)
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