ECTS2016 Poster Presentations Cancer and bone: basic, translational and clinical (37 abstracts)
The clinicopathological implication of GNAS in ewing sarcoma
Byeong-Joo Noh 1 , Ji-Youn Sung 1 , Youn Wha Kim 1 , Eduardo Santini Araujo 2 , Ricardo Karam Kalil 3 , Woon-Won Jung 4 , Hyun-Sook Kim 5 & Yong-Koo Park 1
1Department of Pathology, Kyung Hee University Hospital, Seoul, Republic of Korea; 2Laboratory of Orthopedic Pathology, Buenos Aires, Argentina; 3Surgical Pathology and Electron Microscopy Laboratories, Sarah Network of Rehabilitation Hospitals, Sarah, Brazil; 4Department of Biomedical Laboratory Science, College of Health Science, Cheongju University, Chungbuk, Republic of Korea; 5Department of Biomedical Laboratory Science, College of Health Science, Korea University, Seoul, Republic of Korea.
The objective of our study is to determine whether GNAS expression correlates with pathognomonic signs by analyzing mutations, methylation status, and G-protein α subunit (Gsα) expression of GNAS (guanine nucleotide binding protein/α stimulating) gene in Ewing Sarcoma (ES).
Formalin-fixed paraffin-embedded (FFPE) tissue samples from 77 patients with primary ES were obtained in Korea, Argentina, and Brazil, and were studied via methylation chip assay and direct sequencing of the GNAS gene and immunohistochemical analysis of Gsα. The mutation and methylation statuses of the GNAS gene were examined.
Immunohistochemical results were measured with respect to proportion and staining intensity. We found that GNAS genes in ES tumor samples were less methylated than were normal controls. No mutations were detected at exons 8 or 9 of the GNAS locus complex on chromosome 20q13.3, indicating that the pathogenesis of ES was not associated with GNAS mutation. Gsα expression correlated well with the methylation status of the GNAS gene. Interestingly, high Gsα expression was detected more frequently in samples from living patients than from decedents, although this was not statistically significant (P=0.055).
In conclusion, GNAS mutation is not associated with the pathogenesis of ES tumors. This finding can be used to differentiate ES tumors from metastatic bone lesions with morphological similarity to ES tumors. Analysis of the methylation status of the GNAS gene and immunohistochemical Gsα expression suggests that hypermethylated GNAS gene (low Gsα expression) in ES may be related to unfavorable progression with a non-significant trend.
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Bone Abstracts
ISSN 2052-1219 (online)
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