ECTS2016 Poster Presentations Bone Matrix (4 abstracts)
Cortical bone matrix mineralisation is decreased in TRPV4 deficient male, but not in female mice
Paul Roschger 1 , Barbara Misof 1 , Nadja Fratzl-Zelman 1 , Joost Hoenderop 2 , Rene Bindels 2 , Johannes van Leeuwen 3 , Klaus Klaushofer 1 & Bram van der Eerden 3
1Ludwig Boltzmann Institute of Osteology, Vienna, Austria; 2Radboud University Medical Center, Nijmegen, The Netherlands; 3Erasmus University Medical Center, Rotterdam, The Netherlands.
Transient receptor potential vanilloid channels (TRPVs) have been implicated in Ca2+ homeostasis and bone metabolism. In particular, TRPV4 deficiency was shown to cause sexual dimorphism in bone metabolism and osteoporotic fracture risk. Cortical bone structure was reported to be altered in male TRPV4 knock-out (TRPV4−/−) mice but not in female TRPV−/− mice compared to sex-matched wildtype (TRPV4+/+) animals.
To gain knowledge on this sexual dimorphism, we studied the bone matrix mineralization density distribution (BMDD) based on quantitative backscatter electron imaging in cancellous and cortical femoral bone from six male and six female TRPV4−/− mice as well as from six male and six female TRPV4+/+ mice.
The results from trabecular metaphyseal (MS) and epiphyseal (ES) femoral bone showed that both TRPV4−/− male and TRPV4−/− female mice had a shift of their BMDD to lower calcium concentrations compared to sex-matched TRPV4+/+ animal groups. The typical degree of mineralization CaPeak (the peak position of the BMDD) was reduced in both sexes: MSCaPeak was decreased by −6.3% (P<0.001) and −4.3% (P<0.05) and ESCaPeak by −7.2 and −5.4% (both P<0.01) for male and female mice respectively. In contrast, in diaphyseal cortical (CT) bone, only the male TRPV4−/− revealed lower CTCaPeak −5.8% (P<0.01) compared to male TRPV4+/+ while in the female mice CTCaPeak was not affected by the TRPV4 genotype.
These BMDD findings are a further indication for sexual dimorphism by the TRPV4 genotype. In particular, cortical bone properties including mechanical performance, structure and mineralization are differentially affected in male and female TRPV4−/− mice.
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Bone Abstracts
ISSN 2052-1219 (online)
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