ECTS2016 Poster Presentations Bone development/growth and fracture repair (35 abstracts)
Combination of novel two-photon photopolymerised scaffolds and bioactive elastin-like-recombinamers induce bone regeneration
Carina Kampleitner 1 , Gerhard Hildebrand 2 , Klaus Liefeith 2 , Constancio González 3 , José Carlos Rodriguez-Cabello 3 , Andrea Markus 4 , Christian Dullin 4 , Frauke Alves 4 & Oskar Hoffmann 1
1University of Vienna, Vienna, Austria; 2Institute for Bioprocessing and Analytical Measurement Techniques, Heilbad Heiligenstadt, Germany; 3University of Valladolid, Valladolid, Spain; 4University Medical Center Goettingen, Goettingen, Germany.
Non-healing fractures caused by trauma, disease or tumour resection demand the use of bone grafts to support and stimulate the healing process. We sought to develop and evaluate the effect of a novel bioactive biodegradable biomaterial designed to fill large bone lesions and to improve bone healing. We used two-photon polymerised synthetic polymer scaffolds composed of lactide (LA), caprolactone (CL), and methacrylate (MA) with varying LA: CL ratio and percentage of methacrylation. We tested 3 formulations: 1) LCM3 containing 8 LA: 2 CL and 85% MA; 2) LCM4 containing 9 LA: 1 CL and 90% MA and 3) LCM6.1 containing 9 LA: 1 CL and 40% MA which were either 2D polymer discs or 3D scaffolds and were combined with an injectable thermosensitive elastin-like-recombinamer (ELR) biogel containing the RGD cell adhesion domain and bone morphogenetic proteins (BMP2, BMP7), and hydroxyapatite nanoparticles (HA NPs). We evaluated in vitro mouse osteoblast (OB) and osteoclast (OC) differentiation and cell function and in vivo bone regeneration using a critical-sized calvarial defect (Ø=4 mm) in 8-week old female BALB/c mice. OC development and OB differentiation to mature mineralizing OBs were observed for all scaffold materials. Osteoblastogenesis was supported in the presence of ELRs and results suggest that BMP2 and BMP7-functionalized ELRs simulated OB differentiation. Combined analysis of Quantum FX microCT and histology revealed in vivo cellular attachment associated with osteoconduction. New bone formation was only observed when LCM3 was combined with BMP-containing ELRs. HA NPs enhanced bone regeneration when combined with ELRs and scaffolds. This innovative combination of biomaterials has an osteoinductive effect with potential clinical implications.
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Bone Abstracts
ISSN 2052-1219 (online)
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