ECTS2016 Poster Presentations Bone development/growth and fracture repair (35 abstracts)
Bone regeneration using transcript-activated matrices for sustained messenger RNA delivery
Zohreh Sadat Badieyan 1 , Manish Aneja 2 , Taras Berezhanskyy 2 , Carsten Rudolph 2 & Christian Plank 1,
1Klinikum rechts der Isar, Technical University Munich, Munich, Germany; 2Ethris GmbH, Planegg, Germany.
Transcript therapies, using chemically modified messenger RNAs (cmRNAs), are emerging as safer yet promising substitutes for gene and recombinant protein therapies. However, their applications have been limited due to transient translation and relative low stability of cmRNAs, compared to DNAs. Here we showed that vacuum-dried cmRNA-loaded collagen sponges, so called Transcript-Activated Matrices (TAMs), could serve as depots for sustained cmRNA delivery, providing steady state protein expression for six following days. Another advantage of this technology was high cell transfection efficacy (close to 100%) and low cell toxicity. Considering stability issues, cmRNAs on TAMs were stable at least for 6 months at RT. At the end, bone regeneration in vitro (with MC3T3-E1 cells and MSCs), and in vivo (in rat femur defects), using hBMP2 cmRNAs, confirmed the ability of TAMs in a preclinical application. In total, this study introduces TAMs as stable and efficient sustained cmRNA delivery systems for bone regeneration using hBMP2 cmRNAs.
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Bone Abstracts
ISSN 2052-1219 (online)
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