ECTS2016 Late Breaking Abstracts (1) (18 abstracts)
Rat femoral neck research: an alternative method of histological sections
Marcelo Macedo Crivelini 1 , Érica Araújo de Oliveira 1 , Noélle Egídia Watanabe Kiill 2, , Rita Cassia Menegati Dornelles 2 , João Cesar Bedran de Castro 2 & Wagner Garcez de Mello 3,
1Department of Pathology and Clinical Propaedeutic, School of Dentistry, Univ. Estadual Paulista-UNESP, Araçatuba, São Paulo, Brazil; 2Department of Basic Sciences, Araçatuba Dental School, Univ. Estadual Paulista-UNESP, Araçatuba, São Paulo, Brazil; 3Multicentric Graduate Studies Program in Physiological Sciences, Brazilian Physiological Society/Univ. Estadual Paulista-UNESP, Araçatuba, São Paulo, Brazil; 4Centro Universitário Toledo-UNITOLEDO, Araçatuba, São Paulo, Brazil.
Histomorphometry is often adopted as a methodological approach in research of femoral bone structure. However, it is common do not describe technical details of procedure especially on the steps of macroscopy, paraffin embedding and microtomy. So we propose a simplified, reliable and reproducible method of histological processing for intertrochanteric region and femoral neck of mice, to ensure the achievement of tissue sections with similar structures, cell populations and histometric dimensions. Using a board-guide, femurs of 20 animals (ten males and ten females) aged 120 days were crosswise sectioned in the metaphysis, exactly 2 mm below the line tangent to the lower limit of the femoral head. Standard histological sections were obtained in four equidistant levels (1 mm) involving the plans of: a) the base of the intertrochanteric region; b) intermediate the intertrochanteric region; c) base of the femoral neck (BFN); and d) intermediate region of the femoral neck. CBF was measured by histometry in total bone area, medullary and cortical area, total bone diameter, cancellous bone diameter, and cortical width. Statistically the numerical results demonstrated effectiveness in achieving similar histological sections, with the advantage to view the entire outer edge of the bone tissue and obtain cuts in different tissue levels. In conclusion, this alternative methodology can contribute for the study of bone tissue, ensuring the reproduction of the samples data and the reliability of the histomorphometric results of a research.
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